Log In Sign up
The largest database of trusted experimental protocols

Bone morphogenetic protein 6

Manufactured by R&D Systems
Visit Supplier
Sourced in United States

Bone morphogenetic protein 6 (BMP6) is a recombinant protein that belongs to the transforming growth factor-beta (TGF-β) superfamily. It plays a role in the regulation of bone and cartilage development.

Automatically generated - may contain errors

Lab products found in correlation

Adipogenic induction medium, by Lonza (2 mentions) Toluidine blue, by Fujifilm (2 mentions) Chondrogenic induction medium, by Lonza (2 mentions) Osteogenic induction medium, by Lonza (1 mentions) Alizarin red staining, by Merck Group (1 mentions) Transforming growth factor β3, by Lonza (1 mentions) Oil red o staining, by Muto Pure Chemicals (1 mentions) Trypsin edta, by Thermo Fisher Scientific (1 mentions) Transforming growth factor β3, by R&D Systems (1 mentions) Alizarin red s staining, by Merck Group (1 mentions) Oil red o, by Muto Pure Chemicals (1 mentions) Fetal bovine serum (fbs), by Gemini Bio (1 mentions) Hepg2 cell, by American Type Culture Collection (1 mentions) Sensolyte pnpp alkaline phosphatase assay kit, by AnaSpec (1 mentions)

4 protocols using bone morphogenetic protein 6

1

Multilineage Differentiation of Cultured MSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cultured MSCs at three passages were harvested using Trypsin-EDTA (Gibco). For adipogenic differentiation, 1.0 × 104 cells were transferred to a 24-well plate and cultured overnight in culture medium. Adherent cells were cultured in adipogenic induction medium (Lonza), which was changed every 3–4 days. After 14 days, oil red O staining (Muto Pure Chemicals) confirmed the differentiation of these cells into adipocytes. For osteogenic differentiation, 7.0 × 103 cells were transferred to a 24-well plate and cultured overnight in culture medium. Adherent cells were cultured in osteogenic induction medium (Lonza), which was changed every 3–4 days. After 14 days, the differentiation of these cells into osteoblasts was assessed by alizarin red staining (Millipore). For chondrogenic differentiation, 5.0 × 105 cells were transferred to a 15-mL tube and cultured in chondrogenic induction medium (Lonza) containing 10 ng/mL transforming growth factor-β3 (Lonza) and 500 ng/mL bone morphogenetic protein 6 (R&D Systems), which was changed every 3–4 days. After 21 days, chondrogenic differentiated cells were was analyzed by Toluidine blue (Wako) and Safranin O staining (TCI) staining.
Ogata Y., Mabuchi Y., Yoshida M., Suto E.G., Suzuki N., Muneta T., Sekiya I, & Akazawa C. (2015). Purified Human Synovium Mesenchymal Stem Cells as a Good Resource for Cartilage Regeneration. PLoS ONE, 10(6), e0129096.
+ Open protocol
+ Expand
2

Multilineage Differentiation of Mesenchymal Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
For osteogenic and adipogenic-differentiation, adherent MSCs were cultured in osteogenic or adipogenic induction medium (Lonza), respectively, with twice weekly medium changes. After 21 days differentiation into osteoblasts was confirmed via alkaline phosphatase (ALP)-based enzymatic staining (Vector Laboratories, Burlingame, CA, United States) and Alizarin red S staining (Sigma-Aldrich, St. Louis, MO, United States). After 21 days differentiation into adipocytes was confirmed via Oil red O (Muto Pure Chemicals, Bunkyo, Tokyo, Japan) staining, respectively (Morikawa et al., 2009b (link); Houlihan et al., 2012 (link)).
To induce chondrogenic-differentiation, cultured cells or spheres were transferred into 15-mL tubes containing chondrogenic-induction medium (Lonza) with 10 ng/mL transforming growth factor-β3 (R&D Systems, Minneapolis, MN, United States) and 500 ng/mL bone morphogenetic protein-6 (R&D Systems). The medium was changed twice weekly. After 21 days, chondrogenic-differentiation was confirmed via staining with toluidine blue (Wako) (Morikawa et al., 2009b (link); Houlihan et al., 2012 (link)).
Niibe K., Ohori-Morita Y., Zhang M., Mabuchi Y., Matsuzaki Y, & Egusa H. (2020). A Shaking-Culture Method for Generating Bone Marrow Derived Mesenchymal Stromal/Stem Cell-Spheroids With Enhanced Multipotency in vitro. Frontiers in Bioengineering and Biotechnology, 8, 590332.
+ Open protocol
+ Expand
3

Hepcidin Induction in HepG2 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
HepG2 cells were obtained from American Type Culture Collection (ATCC) and cultured in Eagle’s Minimum Essential Medium (EMEM medium) from ATCC containing 10% Fetal Bovine Serum (FBS) purchased from Gemini Bioproducts. Induction of hepcidin was measured in sub-confluent cultures following replacement of the medium for 48 hours with serum-free EMEM or serum-free EMEM containing 10 ng/ml Bone Morphogenetic Protein 6 (BMP6) (R&D Systems).
Moghieb A., Tesfay L., Nie S., Gritsenko M., Fillmore T.L., Jacobs J.M., Smith R.D., Torti F.M., Torti S.V., Shi T, & Ansong C. (2019). A Targeted Mass Spectrometric Assay for Reliable Sensitive Hepcidin Quantification. Scientific Reports, 9, 7264.
+ Open protocol
+ Expand
4

Osteogenic Differentiation of Bone Marrow Stromal Cells

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Adherent bone marrow stromal cells were cultured to 70% confluency. Cells were then harvested and then seeded at a density of 30,000 cells per cm2. WT cells were maintained in maintenance media (α-MEM supplemented with 10% Fetal bovine serum (FBS), 1% Penicillin-streptomycin, 1% L-glutamine, 1% Non-essential amino acids), osteogenic media (maintenance media + 10 mM β-glycerol phosphate + 50 μM ascorbate-2-phosphate + 100 nM Dexamethasone), osteogenic media in combination with conditioned media collected form either WT or dnMMAL osteoclasts (here condition media was first concentrated using 3 KDa cut off concentration filters (Amicon, Millipore) and then mixed in a ratio of 1:10 with osteogenic media), or osteogenic media in combination with 30 ng/mL Bone Morphogenetic Protein 6 (BMP-6) (R & D Systems). Media were refreshed every 3rd days. Alkaline phosphatase (ALP) activity was measured by SensoLyte pNPP Alkaline Phosphatase Assay Kit (AnaSpec, Inc)39 (link) as per the manufacturer’s instructions. Mineralized nodules were stained with alizarin red as per the protocol described earlier40 (link).
Goel P.N., Moharrer Y., Hebb J.H., Egol A.J., Kaur G., Hankenson K.D., Ahn J, & Ashley J.W. (2019). Suppression of Notch signaling in osteoclasts improves bone regeneration and healing. Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 37(10), 2089-2103.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!