Mice were injected intraperitoneally with 200 μl 3% thioglycolate medium, killed after 3.5 hours and their peritonea washed with 9 ml PBS, 5 mM EDTA. The recovered cells were resuspended in 0.2 ml HBSS, 5 mM EDTA and stained for 1 hour at 4°C for flow cytometry [antibodies used were CD11b-PE (M1/70, eBioscience), Ly6G BV510 (1A8 BD Biosciences), Ly6C-BV605 (AL-21, BD Biosciences), F4/80 BV711 (T45-2342 BD Biosciences), CD4 BV786 (RM4-5 BD Biosciences), CD8-APCCy7 (53-6.7 BD Biosciences), CD19-PE-Cy7 (1D3 BD Biosciences), CD45-PerCP-Cy5.5 (30-F11, BD Biosciences)].
Ly6g bv510
Manufactured by BD
Ly6G BV510 is a fluorochrome-conjugated antibody used for the detection and analysis of Ly6G, a marker for neutrophils, in flow cytometry applications. The BV510 fluorochrome is a bright, tandem dye that can be excited by a violet laser. This product provides a tool for researchers to identify and study neutrophils in their experiments.
Automatically generated - may contain errors
Lab products found in correlation
Cd19 pe cy7, by BD (1 mentions)
Pe cd11b m1 70, by Thermo Fisher Scientific (1 mentions)
Cd8 apc cy7 53 6, by BD (1 mentions)
Cd4 bv786 rm4 5, by BD (1 mentions)
Cd45 percp cy5.5 30 f11, by BD (1 mentions)
Cd3 percp cy5, by BD (1 mentions)
Cd11b pe, by Thermo Fisher Scientific (1 mentions)
Zombie uv fixable viability dye, by BioLegend (1 mentions)
Cd11c pe cy7, by BioLegend (1 mentions)
Cytoflex, by Beckman Coulter (1 mentions)
Cd14 bv605, by BioLegend (1 mentions)
Cd45.2 pe, by Thermo Fisher Scientific (1 mentions)
Cd56 buv737, by BD (1 mentions)
Hla dr buv395, by BD (1 mentions)
Cd19 bv786, by BioLegend (1 mentions)
Cd235a apc cy7, by BioLegend (1 mentions)
2 protocols using ly6g bv510
1
Isolation of Murine Peritoneal Cells
2
Multicolor Flow Cytometry of THP-1 and Blood
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Flow cytometry analysis of THP-1 cells and total blood was performed using the following antibodies. Conjugated anti-human SR-B1 APC (Miltenyi Biotec 130-111-237), CD235a APC Cy7 (BioLegend 3,49,115), CD45 BUV805 (BD Biosciences 6,12,892), CD56 BUV 737 (BD Biosciences 6,12,767), CD11c PeCy7 (BioLegend 3,37,216), CD11b PE (eBioscience 12-0118-41), CD3 PerCP Cy5.5 (BD Biosciences 5,60,835), CD19 BV786 (BioLegend 3,02,239), HLA-DR BUV 395 (BD Biosciences 7,40,302), CD14 BV605 (BioLegend 3,01,834). Labeling of mouse blood was done using the following conjugated antibodies: CD45.2 PE (eBioscience 12-0454–82), CD115 PerCPeF710 (eBioscience 46-1,152-82), CD11b BV650 (eBioscience 48-0112–20), Ly6G BV510 (BD Biosciences 5,63,402), CD3 APC-eF780 (BioLegend 47-0032–82). Fc receptors were blocked using FCR blocking reagent (Myltenyi Biotec). Surface membrane staining was performed in PBS FBS 2%. The Zombie UV fixable viability dye (BioLegend 4,23,107) was used to exclude dead cells. Samples were acquired on a Cytoflex (Beckman Coulter) and analyzed with FlowJo 10 (BD Biosciences).
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