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Psfks y416

Manufactured by Cell Signaling Technology
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The PSFKs Y416 is a laboratory product offered by Cell Signaling Technology. It is a polyclonal antibody that specifically recognizes the phosphorylated form of the Src family kinase activation loop tyrosine residue (Tyr416). This antibody can be used to detect the activation state of Src family kinases through immunodetection techniques.

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Lab products found in correlation

Pakt s473, by Cell Signaling Technology (2 mentions) Pakt t308, by Cell Signaling Technology (1 mentions) Ps6 s240 s244, by Cell Signaling Technology (1 mentions) Pmek s217 221, by Cell Signaling Technology (1 mentions) Integrin antibody sampler kit, by Cell Signaling Technology (1 mentions) Braf antibody, by Santa Cruz Biotechnology (1 mentions) P her3 y1197, by Cell Signaling Technology (1 mentions) Ppdk1 s241, by Cell Signaling Technology (1 mentions) Actin a2066 antibody, by Merck Group (1 mentions) S6 ribosomal protein, by Cell Signaling Technology (1 mentions) Western lightning ecl reagent, by PerkinElmer (1 mentions) E cadherin, by Cell Signaling Technology (1 mentions) Complete protease inhibitor, by Roche (1 mentions) Perk t202 y204, by Cell Signaling Technology (1 mentions) Pegfr y1068, by Cell Signaling Technology (1 mentions) P fak y397, by Cell Signaling Technology (1 mentions) Vimentin, by Cell Signaling Technology (1 mentions) P erk1 2 t202 y204, by Cell Signaling Technology (1 mentions) P p38 t180 y182, by Cell Signaling Technology (1 mentions) Cxcl2 elisa kit, by R&D Systems (1 mentions)

2 protocols using psfks y416

1

Western Blot Analysis of Signaling Proteins

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The following antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA) : EGFR (#2232), pEGFR Y1068 (#2234), HER3 (#4754), pHER3 Y1197 (#4561), pBRAF S445 (#2696), MEK (#9126), pMEK S217/221 (#9154), ERK (#9102), pERK T202/Y204 (#9101), PDK1 (#3062), pPDK1 S241 (#3061), AKT (#9272), pAKT S473 (#9271), pAKT T308 (#9275), ribosomal protein S6 (#2317), pS6 S240/S244 (#5364), FAK (#3285), pFAK Y397 (#8556), SFKs (#2108), pSFKs Y416 (#2101), YES (#3201), Integrin Antibody Sampler Kit (#4749), PARP (#9542), E-cadherin (#3195), Vimentin (#3932), horseradish peroxidase (HRP)-conjugated anti-mouse (#7076) and HRP-conjugated anti-rabbit (#7074). The actin antibody (A2066) was purchased from Sigma-Aldrich. The BRAF antibody (sc-55522) was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). For immunoblot, cells were harvested, washed in PBS, and lysed in RIPA buffer [50 mM Tris•HCl (pH 8.0), 150 mM sodium chloride, 5 mM magnesium chloride, 1% Triton X-100. 0.5% sodium deoxycholate, 0.1% SDS, 40 mM sodium fluoride, 1 mM sodium orthovanadate, and complete protease inhibitors (Roche Diagnostics, Indianapolis, IN, USA)]. Western Lightning ECL reagent (Perkin-Elmer) as used for signal detection. Phosphorylated bands were quantified using ImageJ software.
Ichihara E., Westover D., Meador C.B., Yan Y., Bauer J.A., Lu P., Ye F., Kulick A., de Stanchina E., McEwen R., Ladanyi M., Cross D., Pao W, & Lovly C.M. (2017). SFK/FAK signaling attenuates osimertinib efficacy in both drug-sensitive and drug-resistant models of EGFR-mutant lung cancer. Cancer research, 77(11), 2990-3000.
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2

Multimodal Protein Analysis in Hepatic Disease

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Western blot analyses were performed using standard procedures with the ECL Plus system (Thermo Fisher Scientific). Antibodies targeting the following proteins were used: p-YAP (Y357) (Abcam, ab62751), YAP (Cell Signaling Technology, 4912), p-STAT3 (Y705) (Cell Signaling Technology, 9145), STAT3 (Cell Signaling Technology, 12640), p-SFKs (Y416) (Cell Signaling Technology, 2101), SRC (Thermo Fisher Scientific, AHO1152), Yes (Cell Signaling Technology, 65890), AKT1 (Cell Signaling Technology, 2938), p-AKT (S473) (Cell Signaling Technology, 4060), p38 (Cell Signaling Technology, 8690), p-p38 (T180/Y182) (Cell Signaling Technology, 4511), ERK1/2 (Cell Signaling Technology, 4695), p-ERK1/2 (T202/Y204) (Cell Signaling Technology, 4370), CCN1 (R&D Systems, AF4055), and β-actin (Abcam, ab8226) were detected by the antibodies. The intrahepatic IL-6 and CXCL2 levels were analyzed in the whole liver lysates by using an IL-6 ELISA kit (eBioscience) and CXCL2 ELISA kit (R&D Systems). The serum levels of ALT and AST were measured by the Biological Resources facility at the University of Illinois at Chicago using a Beckman Coulter AU480 analyzer.
Cheng N., Kim K.H, & Lau L.F. (2022). Senescent hepatic stellate cells promote liver regeneration through IL-6 and ligands of CXCR2. JCI Insight, 7(14), e158207.
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