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Mir 338 3p inhibitor

Manufactured by RiboBio
Sourced in China

The MiR-338-3p inhibitor is a laboratory product designed to inhibit the expression of the miR-338-3p microRNA. It can be used as a research tool to study the biological functions and regulatory mechanisms of miR-338-3p in various cellular and molecular processes.

Automatically generated - may contain errors

3 protocols using mir 338 3p inhibitor

1

Transfection of miR-205 and miR-338-3p

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The miR-205 mimics, miR-205 inhibitor, miR-338-3p mimics, and miR-338-3p inhibitor and the negative control were all purchased from Guangzhou RiboBio Co., Ltd. (Guangzhou, China). The cells were transfected using 100 nm Lipofectamine 2000 (Invitrogen Inc., Carlsbad, CA, USA) following the manufacturer’s instructions.
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2

MicroRNA Mimic and Inhibitor Transfection

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The miR-338-3p mimic, miR-338-3p inhibitor, miRNA mimic NC, and miRNA inhibitor NC were purchased from RiboBio (Guangzhou, China). Transfection was carried out using according to the manufacturer’s instructions.
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3

miR-338-3p Regulation in Renal Cell Carcinoma

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RCC cells were seeded into the 6-well plate and were respectively transfected with 5nM miR-338-3p inhibitor, miR-338-3p mimic or miR-control. The Caki-1 and 786-O cells were transfected with Lipofectamine 2000 (Invitrogen). miR-338-3p inhibitor (5′-GCAAAAAUUAGUGUGCGCCAAA-3′), miR-338-3p mimic (5′-UUUGAGCAGCACUCAUUUUUGC-3′) and miR-control (5′-CAGUAC UUUUAGUGUGUACAA-3′) were purchased from RiboBio (Guangzhou, China), the sequence of miR-negative control was heterogenous from any known human genome sequence so as to eliminate the potential non-sequence-specific effect.
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