Lc netii adc interface box

Manufactured by Jasco

Sourced in Italy, Japan

The LC-NetII/ADC interface box is a hardware device designed to connect liquid chromatography (LC) systems to data acquisition and analysis software. Its core function is to serve as an interface between the LC system and a computer, enabling the transfer of data from the LC system for processing and analysis.

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4 protocols using lc netii adc interface box

Quantitative HPLC Analysis of Acyclovir

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A HPLC apparatus equipped with a PU-4180 RHPLC quaternary pump, a UV-4070 UV–Vis, an AS-4050 autosampler and an LC-NetII/ADC interface box (Jasco Europe, Lecco, Italy) was used to assess the analytical quantification of Acyclovir, The samples were eluted under isocratic conditions using an RPC18 column (Mediterranea SEA18 column, size 250 mm × 4.6 mm, 5 µm) operating at room temperature (25 °C) under a flow of 1 mL min⁻¹, and the drug was detected at 251 nm with a retention time of 11,6 min. A mixture of water and acetonitrile (97:3 v/v) containing 0.05% (v/v) of trifluoroacetic acid was filtered through a 0.2 μm polycarbonate filter and used as the mobile phase^{50 (link)}. Data were acquired and processed with ChromNAV v.2.04.01 software (Jasco Inc., Tokyo, Japan), using the linear equation y = 4521.7x + 129,384 with a correlation coefficient (R²) of 0.9999 obtained from the analysis of standard solutions (concentration range of 0.25–25 µg/mL, n = 6). Under the experimental conditions, the limit of detection (LoD) and limit of quantitation (LoQ) were 0.08 µg/mL and 0.25 µg/mL, respectively.
The detection of Methylene Blue was carried out by using a PerkinElmer Lambda 35 ultraviolet–visible (UV–Vis) spectrophotometer at a λmax of 660 nm^{51 (link)}.

Martine T., Antonia M., Cristiano M.C., Konrad U., Daniele T., Donatella P, & Massimo F. (2023). Perspective use of bio-adhesive liquid crystals as ophthalmic drug delivery systems. Scientific Reports, 13, 16188.

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Characterization of Organic Compounds

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The ¹H and ¹³C NMR
spectra were recorded using JEOL JNM-ECX400 and JNM-ECZ600R instruments.
The infrared (IR) spectra were recorded using a Horiba FT-720 spectrometer.
The UV–vis spectra were recorded by a JASCO V-500 spectrophotometer.
The high-resolution mass spectra were recorded by a JEOL AccuTOF JMS-T100LC
(ESI-MS). Aqueous preparative size-exclusion chromatography (SEC)
was performed using ChromNAV software, a JASCO LC-NetII/ADC interface
box, a JASCO FC-2088-30 fraction collector controller, a JASCO PU-2086
Plus pump, a JASCO UV-2075 Plus detector, an Advantec CHF 122 SC fraction
collector, and a Shodex OH pak SB-2002.5 column (20 × 300 mm,
average bead size: 10 μm, exclusion limit: 1 × 10⁴) using water as the eluent at a flow rate of 2.5 mL min^–1 and room temperature.

Narumi A., Rachi R., Yamazaki H., Kawaguchi S., Kikuchi M., Konno H., Osaki T., Okamoto Y., Shen X., Kakuchi T., Kataoka H., Nomoto A., Yoshimura T, & Yano S. (2021). Maltotriose–Chlorin e6 Conjugate Linked via Tetraethyleneglycol as an Advanced Photosensitizer for Photodynamic Therapy. Synthesis and Antitumor Activities against Canine and Mouse Mammary Carcinoma Cells. ACS Omega, 6(10), 7023-7033.

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HPLC Analysis of Nucleobase Sorbent Recovery

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The recovery of each sorbent was evaluated by an HPLC system as follows: a GD-2080-53 degasser, two PU-2085 pumps, an MX-2080-32 dynamic mixer, an AS-4050 autosampler, a CO-2065 Plus column oven, a UV-2070 UV-Vis detector, and an LC-Net II/ADC interface box (Jasco). An InertSustain AQ-C18 (3 μm, 150 × 2.1 mm i.d., GL Sciences) was used for the separation on HPLC. The separation conditions on HPLC were as follows: flow rate, 0.2 mL min -1 ; mobile phase, a 5% methanol solution (for the evaluation of Ura, U, and Ade) or a 10% methanol solution (for the evaluation of A, C, and G); column temperature, 40°C; and injection volume, 10 μL. Elemental analysis was performed by JM11 (J-SCIENCE LAB CO., Ltd.). The specific surface area of each base resin was measured by a Micromeritics TriStar II Surface Area and Porosity Analyzer.

Murakami H., Sugiyama T., Miki Y., Umemura T., Esaka Y., Inoue Y, & Teshima N. (2020). Development and Evaluation of HILIC-type Sorbents Modified with Hydrophilic Copolymers for Solid-phase Extraction. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 36(10).

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Synthesis of UHMW PMMA/[C2mIm][TFSI] Gel

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Here, a representative synthesis of the UHMW PMMA/[C₂mIm][TFSI] gel is described. MMA (0.300 g, 3.00 mmol), [C₂mIm][TFSI] (0.700 g, 1.79 mmol), and AIBN (0.1 mg, 0.61 × 10⁻³ mmol) were charged in a glass vial and sealed with a rubber septum, through which argon was bubbled for 15 min at room temperature. Polymerization was conducted at 80°C for 24 hours. Monomer conversion was determined by ¹H-NMR. The molecular weight and polydispersity index of the polymer were determined by GPC using a 10 mM lithium bromide (LiBr)/N,N-dimethylformamide (DMF) solution as the eluent. The GPC system was equipped with a PU-2080 Plus HPLC pump, a DG-2080-53 3-Line degasser, an RI-4030 refractive index detector, a UV-2075 Plus UV/VIS detector, an LC-NetII/ADC interface box, and a CO-4060 column oven (all equipment is manufactured by JASCO, Japan). Two columns (SB-806M HQ, Showa Denko, Japan), which were kept in a column heater at 40°C, were used for separation and calibrated using PMMA standards. For the characterization by ¹H-NMR and GPC, the as-prepared UHMW gel pieces were dissolved in CDCl₃ (ca. 1 wt %) and 10 mM LiBr/DMF (0.1 wt %) under stirring at room temperature overnight.

Kamiyama Y., Tamate R., Hiroi T., Samitsu S., Fujii K, & Ueki T. (2022). Highly stretchable and self-healable polymer gels from physical entanglements of ultrahigh–molecular weight polymers. Science Advances, 8(42), eadd0226.

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