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Anti atg 12 d88h11

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Anti-ATG 12(D88H11) is a primary antibody that recognizes the ATG 12 protein. ATG 12 is a critical component of the autophagy pathway, which is involved in the degradation and recycling of cellular components. This antibody can be used to detect and analyze the expression and localization of ATG 12 in various cellular and tissue samples.

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Lab products found in correlation

Anti α tubulin, by Merck Group (2 mentions) G box ichemi xt imager, by Syngene (2 mentions) Anti lc3bi 2 3868, by Cell Signaling Technology (2 mentions) Sc 376984, by Santa Cruz Biotechnology (1 mentions) Anti cleaved parp, by Cell Signaling Technology (1 mentions)

2 protocols using anti atg 12 d88h11

1

Western Blot Analysis of Autophagy Markers

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed using 1% Triton in TBS containing protease and phosphatase inhibitors. Tumors were lysed using 1× RIPA buffer containing protease and phosphatase inhibitors. Equal amounts of total protein were separated by electrophoresis on a 4%–12% Bis-Tris gel and transferred to a polyvinylidene difluoride membrane (23 (link)). Blots were blocked in 5% BSA, and then probed with antibodies against anti-NURR1 (sc-376984, Santa Cruz Biotechnology), anti-ATG 7 (10088–2AP, ProteinTech), anti-ATG 12(D88H11, Cell Signaling Technology), anti-LC3BI/II (3868, Cell Signaling Technology), anti-cleaved PARP (9532S, Cell Signaling Technology), and anti-α-tubulin (Sigma). Chemiluminescent (32106, Thermo Fisher Scientific) signal was captured using a Syngene G-BOX iChemi XT imager (26 (link)).
Zarei M., Shrestha R., Johnson S., Yu Z., Karki K., Vaziri-Gohar A., Epps J., Du H., Suva L., Zarei M, & Safe S. (2021). Nuclear Receptor 4A2 (NR4A2/NURR1) Regulates Autophagy and Chemoresistance in Pancreatic Ductal Adenocarcinoma. Cancer Research Communications, 1(2), 65-78.
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2

Western Blot Analysis of Autophagy Markers

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed using 1% Triton in TBS containing protease and phosphatase inhibitors. Tumors were lysed using 1X RIPA buffer containing protease and phosphatase inhibitors. Equal amounts of total protein were separated by electrophoresis on a 4–12% Bis-Tris gel and transferred to a PVDF membrane (23 (link)). Blots were blocked in 5% BSA, and then probed with antibodies against anti- NURR1 (sc-376984, SCB), anti- ATG 7 (10088-2AP, Proteintech), anti-ATG 12(D88H11, Cell Signaling Technology), anti-LC3BI/II(3868, Cell Signaling Technology), anti-Cleaved PARP (9532S, Cell Signaling Technology) and anti-α-tubulin (Sigma). Chemiluminescent (32106, Thermo Fisher Scientific) signal was captured using a Syngene G-BOX iChemi XT imager (26 (link)).
Zarei M., Shrestha R., Johnson S., Yu Z., Karki K., Vaziri-Gohar A., Epps J., Du H., Suva L., Zarei M, & Safe S. (2021). Nuclear Receptor 4A2 (NR4A2/NURR1) Regulates Autophagy and Chemoresistance in Pancreatic Ductal Adenocarcinoma. Cancer Research Communications, 1(2), 65-78.
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