Agilent 1200 series hplc device

For the LC-MS of white flowers of M. denudata, an Agilent 1200 series HPLC device (Agilent Technologies, Palo Alto, CA, USA) coupled to a model G1315D diode array detector (DAD), and a 4000 Qtrap LC/MS/MS system (Applied Biosystems Instrument, Foster City, CA, USA) using the negative ion mode ([M − H]⁻). The LC-MS conditions were set as follows: scan time of 4.80 s; scan range from 100 to 1300 m/z; curtain gas (N₂) at 20.00 psi; nebulizer gas at 50 psi; drying gas at 50 psi; drying gas temperature of 550 °C; entrance potential of 10 V; declustering potential of 100 V; and ionization voltage of 5000 V.

Agilent 1200 series HPLC device (Agilent Technologies, Santa Clara, CA, USA) equipped with an autosampler, thermostatted column compartment, quaternary pump, Diode Array Detector (DAD), and degasse was used. A Grace Apollo C₁₈ column (Alltech, USA, 4.6 mm × 150 mm, 5 μm) was utilized. The column temperature was 25 °C. The detector was DAD detector and the wavelengths were 255 nm and 334 nm. The mobile phase was made up of (A) acetonitrile (Xingke, China) and (B) 0.1% aqueous phosphoric acid (Sinopharm, China) with the gradient elution of 1% to 27% A in 0–80 min, 27% A in 81–95 min, 27%–100% A in 96–110 min, 100%-1% A in 111 min, 1% A in 111–120 min. The flow rate was 0.8 ml/min and the injection volume was 20 μl.