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Nativepage novex 4 16 bis tris

Manufactured by Thermo Fisher Scientific
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The NativePAGE Novex 4-16% Bis-Tris is a pre-cast polyacrylamide gel used for the electrophoretic separation of native, non-denatured proteins. It utilizes a Bis-Tris buffer system and has a gradient concentration of 4-16% acrylamide, allowing for the separation of a wide range of protein sizes.

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Lab products found in correlation

Sc 8334, by Santa Cruz Biotechnology (2 mentions) Pde10a, by Enzo Life Sciences (2 mentions) A00682, by GenScript (2 mentions) Nupage novex 4 12 bis tris, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

NativePAGE Novex 4–16% Bis-Tris Protein Gel NativePAGE™ Novex® 4-16% Bis-Tris gels NativePAGE Novex 4-16% Bis-Tris Gel System NativePAGE™ 4-16% Bis-tris

2 protocols using nativepage novex 4 16 bis tris

1

Western Blot Analysis of Cyclic Nucleotide Phosphodiesterases

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Lysates were run on either denaturing NuPAGE Novex 4-12% Bis-Tris or NativePAGE Novex 4-16% Bis-Tris gels, as per manufacturer’s instruction (Life Technologies). Blots were probed overnight at 4°C with antibodies recognizing PDE11A (1:500, PD11A-112AP FabGennix—Frisco, TX [4 (link)-6 (link)]), PDE2A (1:1000, PD2A-101AP FabGennix [38 (link)] ), PDE10A (1:2500, Enzo Life Science; Farmingdale, NY [39 (link)]), actin (1:10,000, A2066 Sigma; St. Louis, MO), IL-6 (1:200, MAB406 R&D Systems; Minneapolis, MN [40 ]), GFP (1:2000, sc-8334 Santa Cruz; Dallas, TX), or RFP (1:10,000, A00682 Genscript). Blots were then processed as previously published [4 (link), 6 (link), 41 (link)].
Pathak G., Agostino M.J., Bishara K., Capell W.R., Fisher J.L., Hegde S., Ibrahim B.A., Pilarzyk K., Sabin C., Tuczkewycz T., Wilson S, & Kelly M.P. (2016). PDE11A negatively regulates lithium responsivity. Molecular psychiatry, 22(12), 1714-1724.
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2

Western Blot Analysis of Cyclic Nucleotide Phosphodiesterases

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Lysates were run on either denaturing NuPAGE Novex 4-12% Bis-Tris or NativePAGE Novex 4-16% Bis-Tris gels, as per manufacturer’s instruction (Life Technologies). Blots were probed overnight at 4°C with antibodies recognizing PDE11A (1:500, PD11A-112AP FabGennix—Frisco, TX [4 (link)-6 (link)]), PDE2A (1:1000, PD2A-101AP FabGennix [38 (link)] ), PDE10A (1:2500, Enzo Life Science; Farmingdale, NY [39 (link)]), actin (1:10,000, A2066 Sigma; St. Louis, MO), IL-6 (1:200, MAB406 R&D Systems; Minneapolis, MN [40 ]), GFP (1:2000, sc-8334 Santa Cruz; Dallas, TX), or RFP (1:10,000, A00682 Genscript). Blots were then processed as previously published [4 (link), 6 (link), 41 (link)].
Pathak G., Agostino M.J., Bishara K., Capell W.R., Fisher J.L., Hegde S., Ibrahim B.A., Pilarzyk K., Sabin C., Tuczkewycz T., Wilson S, & Kelly M.P. (2016). PDE11A negatively regulates lithium responsivity. Molecular psychiatry, 22(12), 1714-1724.
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