Molecular weights of the 2 propen-1-ol oligomer chains were determined using MALDI-TOF-MS spectrometer from the Bruker Biflex III company (Billerica, MA, USA). 2,5-Dihydroxybenzoic acid (DHB) was used as a matrix.
Maldi tof ms spectrometer
Manufactured by Bruker
Sourced in United States, Germany
The MALDI-TOF-MS spectrometer is a laboratory instrument used for the analysis of biomolecules. It utilizes matrix-assisted laser desorption/ionization (MALDI) to generate ions from samples, which are then detected and analyzed by a time-of-flight (TOF) mass spectrometer.
Automatically generated - may contain errors
Lab products found in correlation
Microflex, by Bruker (2 mentions)
Msp 96 polished steel target plate, by Bruker (1 mentions)
Hplc plus, by Merck Group (1 mentions)
Alpha cyano 4 hydroxycinnamic acid, by Bruker (1 mentions)
Trifluoroacetic acid, by Thermo Fisher Scientific (1 mentions)
96 well plate, by Thermo Fisher Scientific (1 mentions)
Varioskan flash multimode reader, by Thermo Fisher Scientific (1 mentions)
Vertex 70, by Bruker (1 mentions)
Ethanol, by Merck Group (1 mentions)
6 protocols using maldi tof ms spectrometer
1
Determining Molecular Weights of Oligomers
2
MALDI-TOF MS Analysis of VuEI Peptide
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The sample containing VuEI (16 pmol) was spotted onto a MSP 96 polished steel target plate (Bruker Daltonics, Germany) and air-dried at room temperature. The spots were covered with 2 μL of saturated matrix solution (alpha-cyano-4-hydroxycinnamic acid, Bruker Daltonics, Germany) prepared in 50% acetonitrile HPLC Plus (Sigma Aldrich) and 0.1% trifluoroacetic acid (Pierce Biotechnology, USA). The mass spectra were acquired using MALDI-TOF MS spectrometer in a linear positive mode (Microflex, Bruker Daltonics, Germany). Bacterial test standard (BTS, Bruker) was used for instrument calibration. The mass spectra data was analyzed in a range of 2,000 to 20,000 m/z.
3
Listeria monocytogenes Identification Protocol
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Clinical specimens were collected and handled according to standard protocols, and all suspect colonies were identified by standard conventional biochemical method (38 ). The BacT/ALERT 3D(240) blood culture system (Becton Dickinson, Maryland, USA) was used. L. monocytogenes was suspected when facultative anaerobic Gram-positive non-sporulating rods with tumbling motility at room temperature were isolated on sheep blood agar. All identifications were confirmed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry by the direct transfer method using the MALDI–TOF MS spectrometer (Bruker Daltonik) and spectra analyzed with IVD MALDI Biotyper 2.3 and reference library DB-9607 (Bruker Daltonik) (39 (link)). In vitro susceptibility in terms of minimal inhibitory concentrations of penicillin on the L. monocytogenes strains was performed using Epsilometer-test (Liofilchem®), with quality control strain Streptococcus pneumoniae ATCC49619 as the reference strain.
4
Anaerobic and Aerobic Bacterial Identification
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Tonsillar samples were cultured on Columbia agar containing 5% sheep blood and chocolate agar (Laboratorios Britania, Argentina) at 37°C with 5% CO2 for 24 to 48 h. Anaerobes were cultured onto Brucella agar supplemented with hemin and vitamin K under anaerobic conditions. All isolates were identified by MALDI-TOF MS. The isolates were identified by the direct colony on plate extraction method as previously described (8 (link)). Mass spectra were acquired using the MALDI-TOF MS spectrometer in a linear positive mode (Microflex, Bruker Daltonics). Mass spectra were analyzed in a m/z range of 2,000 to 20,000. The MALDI Biotyper library version 3.0 and MALDI Biotyper software version 3.1 were used for bacterial identification.
5
Fluorescence Measurement of Hapten Conjugates
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The measurement of fluorescence was carried out by Varioskan Flash Multimode reader (Thermo Fisher Scientific, USA). 96-well plates were purchased from NUNC (Denmark). Hapten conjugation was verified via infrared spectrometer (Vertex 70, Bruker Optics, USA) and MALDI-TOF-MS spectrometer (autoflex speed, Bruker, USA). The acidity and alkalinity of all solutions were measured by Denver UB-10 UltraBasic meter (Denver Instruments, USA). Throughout the experiment, the immunization program was carried out in the constant temperature incubator with constant temperature control accuracy of 0.1°C.
6
Rapid Identification of Methicillin-Resistant Staphylococci
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All isolates were confirmed as Staphylococci by PCR using 16S RNA primer [14] (link). Methicillin resistance was confirmed by Cefoxitin disk (30 mg) on Mueller-Hinton agar and mecA gene detected by PCR. Colonies were harvested from the Mueller-Hinton agar and suspended in 100 ml of water. One ml of suspension deposited on the plate (Bruker Daltonics, Germany) in 2 replicates, and 1 ml of ethanol (Merck) was added to dry at room temperature. 1 ml of matrix, a-cyano-4-hydroxycinnamic acid (Bruker Daltonics) dissolved in solution [50% acetonitrile, 2.5% trifluoroacetic acid, and 47.5% water] (Sigma-Aldrich, USA). MALDI-TOF-MS was analyzed to distinguish the species within MR-CoNS. MALDI-TOF-MS Spectrometer and FlexControl software (Bruker Daltonics, Germany) were analyzed to detect the species' protein. A score of 2.000-3.000 indicates the specieslevel identification. The score from 1.700 to 1.999 indicates genus-level identification, and a score of <1.700 was unreliable [15, 16] (link).
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