Double beam uv visible spectrophotometer 1700

The photosynthetic pigments content were assayed by the method of Lichtenthaler (1987) (link) by recording the absorbance at 663, 646, and 470 nm in spectrophotometer (Shimadzu double beam UV–Visible spectrophotometer-1700, Japan).

Melting points were recorded on a STUART SCIENTIFIC SMP10 instrument. The UV spectra were recorded on a Double beam UV-Visible Spectrophotometer 1700 (SHIMADZU Japan). ¹H- and ¹³C–NMR spectra were recorded on an AVANCE III Bruker Spectrometer at 600 and 150 MHz, respectively. TMS was used as internal standard. ESI-MS spectra were taken on Bruker microTOF-Q spectrometer. All solvents from the extracts were evaporated to dryness using a BUCHI rotary evaporator R-200, water bath and freeze dryer (ALPHA 1–4 LD-2). The dried plant material was pulverised using Fritsch Universal Cutting Mill-Pulverisette 19-Germany. TECAN micro detection microplate reader (M 200) was used to measure the absorbance. Perkin Elmer Multi label HTS reader was used for detecting fluorescent absorbance of 2-NBDG. Evos Microscope and Dino Eye were used for taking pictures of the cells.