For cell marker expression analysis, the HUVEC monolayers were washed with PBS with calcium and magnesium, fixed with 4% PFA before blocking with 10% goat serum (Thermo Fischer Scientific). The monolayers were stained with VE-cadherin (rabbit anti-VE-cadherin, 1:200, Cell Signaling Technology) and ICAM-1 (mouse anti-ICAM-1, 1:200, R&D Systems) and then secondary antibodies (Alexa Fluor™ 488 goat anti-rabbit and Alexa Fluor™ 546 goat anti-mouse), with DAPI as counter staining for the nuclei. Images were captured from each sample (CD31, VE-cadherin, and ICAM-1) with consistent image acquisition parameters, processed with Image J (Image J 1.47v) and evaluated for the endothelial cell markers.
Mouse anti icam 1
Manufactured by R&D Systems
Sourced in United Kingdom
Mouse anti-ICAM-1 is a monoclonal antibody that binds to the cell surface molecule ICAM-1 (Intercellular Adhesion Molecule-1). ICAM-1 is a cell adhesion protein involved in the immune response and inflammatory processes. This antibody can be used in various immunoassays and research applications to detect or study ICAM-1 expression.
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Lab products found in correlation
Goat serum, by Thermo Fisher Scientific (1 mentions)
Anti ve cadherin, by Cell Signaling Technology (1 mentions)
Ve cadherin, by Cell Signaling Technology (1 mentions)
Icam 1, by R&D Systems (1 mentions)
Abc elite, by Vector Laboratories (1 mentions)
Goat anti gfap, by Abcam (1 mentions)
Mouse anti mbp, by Merck Group (1 mentions)
Rabbit anti parvalbumin pv, by Abcam (1 mentions)
Lycopersicon esculentum tomato lectin, by Vector Laboratories (1 mentions)
2 protocols using mouse anti icam 1
1
Endothelial Cell Marker Expression Analysis
2
Immunostaining Techniques for Tissue Analysis
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Immunostaining was performed using an avidin–biotin–peroxidase (ABP) method, with 3,3′-diaminobenzidine (DAB) for visualisation. Sections were incubated overnight (4 °C) in one of the following primary antibodies, diluted in 1% NHS block: Goat anti-serum albumin (1:5000, Abcam), rabbit anti-parvalbumin (PV, 1:200, Abcam), mouse anti-MBP (1:200, Millipore), mouse-anti-CNPase (1:200, Neomarkers), mouse anti-ICAM-1 (1:200, R&D Systems), goat anti-GFAP (1:300, Abcam) and Lycopersicon esculentum (tomato) lectin (1:200, Vector, UK). Biotinylated secondary antibodies (1:200, Vector, diluted in 1% NHS block, 2 h, RT) were used, followed by HRP (ABC Elite, Vector, UK) and DAB prior to imaging and analysis.
For assessment of general tissue structure, sections underwent staining with haematoxylin and eosin (H&E).
For assessment of general tissue structure, sections underwent staining with haematoxylin and eosin (H&E).
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