Cells were lysed and processed as described previously (Pannu et al., 2006 (link)), using anti-type I collagen (SouthernBiotech, Birmingham, AL), anti-phosho-Smad1/5 (Ser463/465) (41D10; Cell Signaling TECHNOLOGY, Danvers, MA), anti-Smad1 (Cell Signaling Technology), anti-phospho-Smad2 (Ser465/467) (Cell Signaling Technology), anti-Smad2/3 (Cell Signaling Technology), anti-CTGF (L-20; Santa Cruz Biotechnology, Dallas, TX), anti-YAP/TAZ (D24E4; Cell Signaling Technology), anti-phospho-GSK3β (Ser9) (119A11; Cell Signaling Technology), anti-GSK3β (H-76; Santa Cruz Biotechnology), anti-phospho-Akt (Ser473) (193H12; Cell Signaling Technology), anti-phospho-YAP (S127) (D9W2I; Cell Signaling Technology) and anti-Akt (C67E7; Cell Signaling Technology) antibodies. Densitometric quantification was done using Image J.
Anti yap taz d24e4
Manufactured by Cell Signaling Technology
The Anti-YAP/TAZ (D24E4) product is a laboratory reagent designed for research purposes. It is a rabbit monoclonal antibody that recognizes the YAP and TAZ proteins. The antibody can be used to detect and analyze the expression of these proteins in various experimental systems.
Automatically generated - may contain errors
Lab products found in correlation
Anti smad1, by Cell Signaling Technology (1 mentions)
Anti smad2 3, by Cell Signaling Technology (1 mentions)
Anti type 1 collagen, by Southern Biotech (1 mentions)
Anti akt c67e7, by Cell Signaling Technology (1 mentions)
Anti phospho smad2 ser465 467, by Cell Signaling Technology (1 mentions)
Anti phospho akt ser473, by Cell Signaling Technology (1 mentions)
Anti phospho gsk3β ser9, by Cell Signaling Technology (1 mentions)
Peroxidase conjugated goat anti mouse igg, by Jackson ImmunoResearch (1 mentions)
Phalloidin tetramethylrhodamine b isothiocyanate, by Merck Group (1 mentions)
Alexa fluor 647 goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Peroxidase conjugated goat anti rabbit igg, by Jackson ImmunoResearch (1 mentions)
2 protocols using anti yap taz d24e4
1
Protein Expression and Quantification
2
Immunofluorescence Analysis of Cell Signaling
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Primary antibodies used in this study included; rabbit monoclonal antibody anti-YAP/TAZ (D24E4; Cell Signaling Technology, Catalog No-8418), rabbit polyclonal anti-TKS5 antibody (Santa Cruz Biotechnology, Catalog No: SC-7390), and rabbit polyclonal anti-TKS5 antibody (Merck, Catalog No: 09-403). Secondary antibodies used here included; peroxidase-conjugated goat anti-mouse IgG (Jackson ImmunoResearch Laboratories, Catalog No: 115-035-003), peroxidase-conjugated goat anti-rabbit IgG (Jackson ImmunoResearch Laboratories, Catalog No: 111-035-144), and goat anti-rabbit IgG Alexa Fluor647 (Thermo Fisher Scientific, Catalog No: A32728). F-actin was stained using Phalloidin-tetramethylrhodamine B isothiocyanate (Sigma Aldrich, Catalog No: P1951). Nuclei were stained using 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; Sigma Aldrich-Aldrich, Catalog No- D9542).
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