To investigate the metabolic state of the hearts, lipid depositions in myocardia were assessed by performing ORO staining described by the manufacturer (Solarbio; G1261). Lipid depositions were observed at X40 magnification and quantified with ImageJ.
G1261
Manufactured by Solarbio
Sourced in China
G1261 is a laboratory instrument designed for the determination of the glucose concentration in various biological samples. It utilizes the glucose oxidase method to measure the glucose levels accurately and reliably.
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Lab products found in correlation
4 protocols using g1261
1
Lipid Deposition Quantification in Myocardium
2
Adipocyte Lipid Deposition Analysis
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To analyze lipid deposition in adipocytes, Oil O staining (G1262, Solarbio, China) was performed for the different groups. To analyze lipid deposition in the human subepicardial layer, modified Oil O staining (G1261, Solarbio, China) was performed. Images were captured using a light microscope (ECLIPSE E100; Nikon, Tokyo, Japan).
3
Visualization of Neutral Lipids in Frozen Sections
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To visualize neutral lipids, primarily triacylglycerol and cholesterol esters, frozen sections were rinsed with 60% isopropanol for 30 s and incubated with freshly prepared ORO working solution (G1261; Solarbio) for 15 min. Next, the slides were differentiated in 60% isopropanol, rinsed with distilled water, and mounted for further imaging.
4
Histological Analysis of Liver Tissue
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After mice received an MCD diet or control diet for 4 weeks, liver tissues were harvested and fixed using 4% paraformaldehyde. Then, 8 μm-thick sections were prepared with a microtome (Leica Biosystems, Nussloch, Germany). After dewaxing and rehydrating, the tissue sections were stained with H&E using a commercial kit (Solarbio, Beijing, China). Microscopic images were obtained using a microscope (Olympus, Tokyo, Japan). For Oil Red O stain, the fixed liver tissues were embedded with OCT and sliced into 8 μm-thick sections. After washing in distilled water, slices were soaked in 60% isopropyl alcohol for 20-30 s. Then, the slices were placed in a dyeing tank filled with a modified oil red O staining solution (G1261, Solarbio Life Sciences) and stained for 10–15 min in the dark. After separating and washing, the slices were counterstained with hematoxylin solution for 1–2 min to reveal the nuclei. Finally, slices were mounted with the glycerin gelatin for observation.
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