Intestinal sections and lungs were harvested and snap-frozen in OCT as above. The cryo-sections (6 μm) were fixed with 10% neutral buffered formalin solution (Sigma) for 10 min at room temperature (RT), rehydrated in PBS, then blocked with 10% normal goat (Vector Labs) or donkey (Abcam) serum with 0.3% Triton X-100 (Sigma) for 20 min at room temperature (RT). The tissues were incubated with primary antibodies (EpCAM-FITC, Biolegend; rabbit anti-mouse ZO-1, Abcam; rabbit polyclonal anti-MUC2, Abcam) overnight at 4°C. The secondary antibodies (goat anti-rabbit IgG-Alexa Fluor 555, Thermo Fisher, donkey anti-rabbit IgG-Alexa Fluor 488, Thermo Fisher) were added the following day after X2 PBS washes and incubated for 1 hour at RT. The slides were finally mounted in Prolong Antifade Mountant with DAPI (Thermo Fisher) or Vectashield with DAPI (Vector Labs), imaged on a Zeiss fluorescence microscope using AxioVision or Zen lite (Blue edition) software and analyzed using Fiji (ImageJ) for mean fluorescence intensity.
Rabbit anti mouse zo 1
Manufactured by Abcam
Sourced in United States
Rabbit anti-mouse ZO-1 is a primary antibody that specifically binds to the zonula occludens-1 (ZO-1) protein in mouse samples. ZO-1 is a tight junction-associated protein that plays a role in regulating epithelial and endothelial cell permeability. This antibody can be used for various applications, such as Western blotting, immunohistochemistry, and immunocytochemistry, to detect and study the ZO-1 protein in mouse-derived samples.
Automatically generated - may contain errors
Lab products found in correlation
Vectashield with dapi, by Vector Laboratories (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Alexa fluor 488 donkey anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Epcam fitc, by BioLegend (1 mentions)
Prolong antifade mountant with dapi, by Thermo Fisher Scientific (1 mentions)
Neutral buffered formalin solution, by Merck Group (1 mentions)
Goat anti rabbit igg alexafluor 555, by Thermo Fisher Scientific (1 mentions)
Goat anti rabbit igg, by Abcam (1 mentions)
Eclipse 80i microscope, by Nikon (1 mentions)
2 protocols using rabbit anti mouse zo 1
1
Immunofluorescent Tissue Staining Protocol
2
Immunofluorescence Analysis of Tight Junction Proteins
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B4G12 cells were triple washed with prewarmed phosphate-buffered saline (PBS) and fixed in 4% paraformaldehyde at room temperature for 20 min. B4G12 cells were blocked with 5% bovine serum albumin in PBS for 1 h at room temperature and then incubated with primary antibody (rabbit anti-mouse Claudin-5, 1:50, and rabbit anti-mouse ZO-1, 1:100, Abcam, MA, USA) at 4 °C overnight. Cells were washed three times with PBS and incubated with secondary antibody (goat anti-rabbit IgG, 1:100, Abcam, USA) at room temperature in the dark for 1 h. The cell nucleus was stained using DAPI. The slide was sealed and observed with Nikon Eclipse 80i microscope (Nikon, Tokyo, Japan).
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