Male sprague dawley cd young adult rats

Male Sprague Dawley CD young-adult rats (Charles River, Como, Italy) were bred in our animal facility and maintained under an artificial 12 h-light, 12 h-dark cycle (lights on from 08:00 to 20:00 h), at a constant temperature of 22 ± 2°C, and a relative humidity of 65%. They had free access to water and standard laboratory food at all times. All efforts were made to minimize animal suffering. Animal care and handling throughout the whole experimental procedures were made in accordance with the European Communities Council Directive of 24 November 1986 (86/609/EEC). The experimental protocols were also approved by the Animal Ethics Committee of the University of Cagliari and by the Italian Ministry of Health (authorization #353/2015-PR).

Male Sprague Dawley CD young-adult rats (Charles River, Como, Italy) were bred in our animal facility and maintained under an artificial 12-h-light, 12-h-dark cycle (lights on from 08∶00 to 20∶00 hours), a constant temperature of 22° ±2°C, and a relative humidity of 65%. They had free access to water and standard laboratory food at all times until the food restriction regimen was applied. All efforts were made to minimize animal suffering. Animal care and handling throughout the whole experimental procedures were made in accordance with the European Communities Council Directive of 24 November 1986 (86/609/EEC). The experimental protocols were also approved by the Animal Ethics Committee of the University of Cagliari.