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2 protocols using anti zfp207

1

Western Blot and Immunofluorescence Antibody Validation

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The following commercially available antibodies were used at the indicated concentrations for western blot: Anti‐ZFP207 (Santa Cruz Biotechnology, sc‐271943, 1:500), Anti–β‐ACTIN (Sigma‐Aldrich, A5441, 1:2,500), Anti‐OCT3/4 (Santa Cruz Biotechnology, sc8628, 1:2,500), Anti‐Nanog (Santa Cruz Biotechnology, sc‐374001, 1:1,000), Anti‐Sox2 (Santa Cruz Biotechnology, sc‐398254, 1:1,000), Anti‐SFRS11 antibody (Abcam, ab196801, 1:2,000), Goat Anti‐Rabbit IgG H&L (HRP) (Abcam, ab6721, 1:5,000), Goat Anti‐Mouse IgG H&L (HRP) (Abcam, 1:1,000, ab6789), Rabbit Anti‐Goat IgG H&L (HRP) (Abcam, 1:5,000, ab6741), and Rabbit Anti‐ goat IgG (HRP) (Abcam, ab6771, 1:5,000), Anti‐Flag (Sigma, F3165, 1:1,000). In all western blots, β‐ACTIN was used as a loading control (Sigma, A5441, 1:2,500). For IF staining, we used Anti‐SSEA1 (Invitrogen, MA5‐17042, 1:250), Anti‐Nestin (Abcam, ab81462, 1:50), Anti‐Tuj1 (Abcam, ab18207, 1:200), Anti‐Caspase 3 Antibody, active (cleaved) form (MERK, AB3623, 1:100), Goat anti‐mouse IgG AF488 (Invitrogen, A11029, 1:1,000), Goat anti‐rabbit IgG AF568 (Invitrogen, A11011, 1:1,000), Anti‐Mouse IgG HRP (Abcam, ab6789, 1:1,000), and Donkey Anti‐Goat IgG AF594 (Invitrogen, A11058, 1:250).
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2

Antibody Panel for Stem Cell Characterization

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The following commercially available antibodies were used at the indicated concentrations for western blot: Anti-ZFP207 (Santa Cruz Biotechnology, sc-271943, 1:500), anti-β-ACTIN (Sigma-Aldrich, A5441, 1:2500), Anti-LAMIN A/C (Abcam ab108922), Anti-OCT3/4 (Santa Cruz Biotechnology, sc8628, 1:2500), Goat Anti-Rabbit IgG H&L (HRP) (Abcam, ab6721, 1:5000), Goat Anti-Mouse IgG H&L (HRP) (Abcam, 1:1000, ab6789), Rabbit Anti-Goat IgG H&L (HRP) (Abcam, 1:5000, ab6741), and Rabbit Anti-goat IgG (HRP) (Abcam, ab6771, 1:5000). For IF staining, we used Anti-SSEA1 (ThermoFisher Scientific, MA5-17042, 1:250), Anti-Nestin (Abcam, ab6142, 1:50), Anti-Tuj1 (Abcam, ab18207, 1:200), Anti-OCT3/4 (Santa Cruz Biotechnology, sc8628, 1:2500), Nucleolin (Abcam, ab50279, 1:130), Goat anti-mouse IgG AF488 (ThermoFisher Scientific, A11029, 1:1000), Goat anti-rabbit IgG AF568 (ThermoFisher Scientific, A11011, 1:1000), Anti-Mouse IgG HRP (Abcam, ab6789, 1:1000), and Donkey Anti-Goat IgG AF594 (ThermoFisher Scientific, A11058, 1:250).
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