Non-cytotoxic confirmed S. neurona-inhibitory compounds were purchased for additional testing. Altanserin, chloroxine, diphenylcyclopropenone, and pyrimethamine were purchased from Sigma. Thiothixene and 5-fluorouracil were purchased from AK Scientific. Perospirone HCl was purchased from Carbosynth. AM-251, artesunate, azelastine HCl, carmofur, clofazimine, dantrolene, disulfiram, hexachlorophene, perphenazine, prazosin, and primaquine phosphate were purchased from TargetMol. Purchased chemicals were used to prepare 10 mM stock solutions in 100% DMSO. Assay plates seeded with BT cells and infected with 2.0 × 104S. neurona + GFP/FLUC parasites were treated with growth media supplemented with 10, 5, 2.5, 1, 0.5, 0.1, and 0.5 μM compound or 0.1% DMSO control. Compounds were added to assay plates in triplicate and incubated at 37 °C and 5% CO2 for 4 days before measuring FLUC activity as before. Estimation of EC50 value for each compound was accomplished using a four-parametric logistic function of GraphPad Prism 7.00 Software (GraphPad, USA).
Perphenazine
Manufactured by Targetmol
Sourced in China
Perphenazine is a phenothiazine derivative used in the laboratory setting. It is a chemical compound with the molecular formula C21H26ClN3S. Perphenazine functions as a neuroleptic agent and is commonly used in research applications.
Automatically generated - may contain errors
Lab products found in correlation
Chloroxine, by Merck Group (1 mentions)
Pyrimethamine, by Merck Group (1 mentions)
Prazosin, by Targetmol (1 mentions)
Prism 7, by GraphPad (1 mentions)
Altanserin, by Merck Group (1 mentions)
Artesunate, by Targetmol (1 mentions)
Am251, by Targetmol (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Hek293t, by American Type Culture Collection (1 mentions)
Huh7 cell line, by American Type Culture Collection (1 mentions)
2 protocols using perphenazine
1
Screening Non-Cytotoxic Compounds Against S. neurona
2
Characterization of HEK293T and Huh 7 Cell Lines
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The human embryonic kidney cell line 293T (HEK293T) and human hepatoma Huh 7 cell line were purchased from American Type Culture Collection (ATCC). The HEK293T-hACE2 cell line was established by infecting HEK293T cells with lentiviruses that expressed hACE2. All the cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco, NY, USA) supplemented with 10% fetal bovine serum (FBS), 100 units/mL penicillin, 100 μg/mL streptomycin, and 2% L-glutamine (Gibco). All cells have been regularly monitored for detecting mycoplasma by a PCR-based assay and confirmed to be mycoplasma-free.
The compounds including perphenazine, fluphenazine decanoate, acepromazine maleate, prochlorperazine maleate, and alimemazine hemitartrate were purchased from TargetMol (Shanghai, China) and dissolved in dimethylsulfoxide (DMSO) and stored at −80 °C.
The compounds including perphenazine, fluphenazine decanoate, acepromazine maleate, prochlorperazine maleate, and alimemazine hemitartrate were purchased from TargetMol (Shanghai, China) and dissolved in dimethylsulfoxide (DMSO) and stored at −80 °C.
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