For the cell growth assay, 2 × 103 (BT549, MDA-MB-231, and MCF7) and 4 × 103 (T47D, SKBR3, and BT474) cells were preseeded in 96-well plates in triplicate and incubated with 10% Cell Counting Kit-8 (CCK-8) solution (Vazyme, #A311-02) at 37 °C for 2 h, and then the absorbance was measured at 450 nm using a microplate reader. For the colony formation assay, 1 × 103 BT549 cells and 2 × 103 MDA-MB-231 cells were seeded into 6-well plates in triplicate, fixed after 12 days, and stained with 0.25% crystal violet staining solution. Colonies consisting of more than 50 cells were counted.
Cell counting kit 8 cck 8 solution
Manufactured by Vazyme
Sourced in China
The Cell Counting Kit-8 (CCK-8) solution is a colorimetric assay for the determination of the number of viable cells in cell proliferation and cytotoxicity assays. The kit utilizes a tetrazolium salt that is reduced by dehydrogenases in viable cells, producing a water-soluble formazan dye that can be detected spectrophotometrically.
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Lab products found in correlation
2 protocols using cell counting kit 8 cck 8 solution
1
Cell Growth and Colony Formation Assays
2
Cytotoxicity Assay of Purp@COP in HepG2 Cells
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The tumor cell line HepG2 was uniformly seeded on a 96-well plate with an attachment time of 24 h. Cells were cultured with new DMEM and treated with Purp@COP at the following concentrations: 0, 10, 20, 50, and 100 μg/ml. After 24 h of incubation in the incubator, 10 μl of Cell Counting Kit-8 (CCK-8) solution (Vazyme, Nanjing, China) was added to each well and incubated for 1.5 h at 37°C. The 96-well plate was measured at 450 nm according to a microplate reader (spectraMax-i3). The optical density of each well was normalized and calculated.
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