Sephadex G-200 (discontinued product) was originally obtained from Pharmacia Biotech (now GE Healthcare). The Microsource Spectrum Collection and small-molecule library containing 2560 compounds included in the US and International Drug Collections, together with Natural Product and Discover libraries, was purchased from Microsource Discovery Systems, Inc. DMSO and H2O2 (30%) were from Thermo Fisher Scientific. DTPA and all other chemicals were from Sigma–Aldrich and were from the highest available purity grade. Specialty chemicals (>95% purity guarantee by manufacturer) present in the Microsource library that were purchased in powder form included the following: gambogic acid (drug G) from Shanghai Tauto Biotech Co, closantel (drug C), hematoporphyrin (drug H), hexachlorophene (drug X), and docusate sodium (drug DS) from MCE (MedChemExpress), dihydrogambogic acid (drug D), chaulmoogric acid (drug M) and hematein (drug I) from MicroSource, Inc, bixin (drug B), sennoside A (drug S), tetradecyl sulfate sodium (drug T), agaric acid (drug A) and escin (drug E) from Sigma, avocadene (drug V) and all other drugs were taken directly from the Microsource microtiter plate library as 10 mM solutions in DMSO.
Agaric acid
Manufactured by Merck Group
Agaric acid is a chemical compound that is commonly used in laboratory settings. It is a naturally occurring carboxylic acid derived from certain species of fungi. Agaric acid serves as a key component in various analytical and experimental procedures conducted in scientific research and development.
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2 protocols using agaric acid
1
Sephadex G-200 Purification Protocol
2
Microdilution Assay for Agaric Acid Inhibition
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MIC values were determined in a 96-well plate. Two-fold serial dilutions of agaric acid (Sigma) or dimethyl sulfoxide (DMSO) were prepared in 100 μl of TSB 1/20 and 100 μL of the inoculum diluted 1/100 in TSB 1/20 was added. The plate was covered with a breathable sealing membrane and a lid and incubated for 24 h at 25 °C, shaking at 200 rpm. The MIC was defined as the lowest concentration of compound were Salmonella growth was lower than the upper bound of the 95% confidence interval of the negative control.
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