Human osteosarcoma MG63 and normal osteoblast hFOB cell lines were purchased from the Cell Bank Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). Dulbecco's modi ed Eagle's medium-F12 (DMEM/F12) was purchased from Hyclone, USA; Fetal Bovine Serum (FBS), Penicillin-streptomycin (100 U/mL) were purchased from Hangzhou Sijiqing Biological Company; RIPA Lysis buffer, BCA Protein Quanti cation Reagent Kit were purchased from Invitrogen, USA; Opti-MEM (Gbico, UK); Anti-Tim-3 monoclonal antibody was obtained from BD Bioscience, USA; Rabbit anti-E-Cadherin, N-cadherin, Vimentin, and GAPDH monoclonal antibodies were purchased from Abcam, USA; Rabbit anti-CD9, CD81, and CD63 monoclonal antibodies were purchased from Santa Cruz, USA; Horseradish peroxidase (HRP) labeled goat anti-rabbit IgG secondary antibody was purchased from Guangzhou Jingcai; PKH67 green uorescent cell linker dye, and PMA were purchased from Sigma Company, USA.
Pkh67 green uorescent cell linker dye
Manufactured by Merck Group
Sourced in United States
PKH67 is a green fluorescent cell linker dye. It is a lipophilic compound that binds to the cell membrane, allowing the labeling of cells without affecting their viability or functionality.
Automatically generated - may contain errors
Lab products found in correlation
Ripa lysis buffer, by Thermo Fisher Scientific (2 mentions)
Bca protein quanti cation reagent kit, by Thermo Fisher Scientific (2 mentions)
Anti tim 3 monoclonal antibody, by BD (2 mentions)
Opti mem, by BD (2 mentions)
Rabbit anti e cadherin, by Abcam (2 mentions)
Phorbol myristate acetate (pma), by Merck Group (2 mentions)
Vimentin, by Abcam (2 mentions)
N cadherin, by Abcam (2 mentions)
2 protocols using pkh67 green uorescent cell linker dye
1
In vitro Study of Human Osteosarcoma
2
In vitro Study of Human Osteosarcoma
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Human osteosarcoma MG63 and normal osteoblast hFOB cell lines were purchased from the Cell Bank Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). Dulbecco's modi ed Eagle's medium-F12 (DMEM/F12) was purchased from Hyclone, USA; Fetal Bovine Serum (FBS), Penicillin-streptomycin (100 U/mL) were purchased from Hangzhou Sijiqing Biological Company; RIPA Lysis buffer, BCA Protein Quanti cation Reagent Kit were purchased from Invitrogen, USA; Opti-MEM (Gbico, UK); Anti-Tim-3 monoclonal antibody was obtained from BD Bioscience, USA; Rabbit anti-E-Cadherin, N-cadherin, Vimentin, and GAPDH monoclonal antibodies were purchased from Abcam, USA; Rabbit anti-CD9, CD81, and CD63 monoclonal antibodies were purchased from Santa Cruz, USA; Horseradish peroxidase (HRP) labeled goat anti-rabbit IgG secondary antibody was purchased from Guangzhou Jingcai; PKH67 green uorescent cell linker dye, and PMA were purchased from Sigma Company, USA.
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