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D glucose 6 phosphate disodium salt hydrate g 6 p

Manufactured by Merck Group
Sourced in Poland, United States

D-glucose 6-phosphate disodium salt hydrate (G-6-P) is a chemical compound that serves as a laboratory reagent. It is a salt of glucose 6-phosphate, which is an important intermediate in cellular metabolism. G-6-P is commonly used in enzymatic assays and other biochemical applications.

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3 protocols using d glucose 6 phosphate disodium salt hydrate g 6 p

1

Enzymatic Activity Assessment Assay

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2-aminoanthracene (2AA) and chloropromazine (CPZ) were purchased from Sigma-Aldrich (Poznań, Poland). 2AA were dissolved in DMSO. CPZ was dissolved in deionized water. D-glucose 6-phosphate disodium salt hydrate (G-6-P) (CAS no. 3671-99-6), disodium salt hydrate (CAS no. 3671-99-6), and 2-nitrophenyl β-d-galactopyranoside (ONPG) (CAS no. 369-07-3), which was the β-galactosidase enzyme, were purchased from Sigma Aldrich. Nicotinamide adenine dinucleotide phosphate (NADP) (CAS no. 24292-60-2) was purchased from MP Biomedicals (Irvine, CA, USA). DMSO (CAS no. 67-68-5) was purchased from Avantor Performance Materials (Gliwice, Poland). Methanol (CAS no. 67-56-1) was purchased from Merck (Warsaw, Poland).
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2

Prostate Cancer Cell Line Assay

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LNCaP human prostate cancer cell line was obtained from ATCC (Manassas, VA, USA). Salmonella typhimurium strain TA1535/pSK1002 was purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ, Inhoffenstraße 7B 38124 Braunschweig, Germany). DMEM medium supplemented with phenol red, Ultraglutamine and high glucose (4.5 g/L) was from Lonza (Verviers, Belgium). The solutions of penicillin/streptomycin, 0.25% trypsin-EDTA and phosphate buffered saline (PBS) were from Gibco (Paisley, UK). Genistein (≥98%, HPLC), daidzein (≥98%), equol (≥99%, TLC), fetal bovine serum (FBS), sodium pyruvate solution (100 mM), deoxyribonucleic acid sodium salt type XIV: from herring testes, bisbenzimide H 33258, camptothecin, 4-nitroquinoline N-oxide (4NQO), 2-aminoanthracene (2AA), d-glucose 6-phosphate disodium salt hydrate (G-6-P) and 2-nitrophenyl β-d-galactopyranoside (ONPG) were purchased from Sigma-Aldrich (Poznań, Poland). Nicotinamide adenine dinucleotide phosphate (NADP) was purchased from MP Biomedicals. Zinc sulphate (ZnSO4 × 7H2O, ≥99.5%), sodium selenate (Na2SeO4, ≥98%), copper(II)/cupric sulphate (CuSO4 × 5H2O, ≥99%), iron(II) sulphate (FeSO4 × 7H2O, ≥99%), DMSO were obtained from POCH (Gliwice, Poland) and calcium chloride (CaCl2 × 6H2O, ≥98%) was from Chempur (Piekary Śląskie, Poland). Methanol was purchased from Merck (Warsaw, Poland).
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3

Fosfomycin and Colistin Dilution Protocol

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Fosfomycin powder (96.5 % purity) was obtained from the National Institute for the Control of Pharmaceutical and Biological Products (NICPBP, Beijing, China). Colistin sulfate powder with a potency of 23 584 U mg -1 and D-glucose-6-phosphate disodium salt hydrate (G-6-P) were purchased from Sigma-Aldrich (St Louis, MO, USA). Mueller Hinton agar (MHA) and cationadjusted Mueller II broth (CA-MHB) were purchased from Becton, Dickinson (Franklin Lakes, NJ, USA). Fosfomycin and colistin were diluted in sterile water and used as prepared stock solutions of 2048 μg ml -1 and 1024 μg ml -1 , respectively.
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