After NG2 sorting, cells were pelleted and RNA was extracted using RNeasy Micro Kit (QIAGEN) according to manufacturer's instructions. RNA integrity was assessed by RNA integrity number (RIN) values as shown in Supplementary Table 1 . NGS library prep was performed with NuGen Ovation SoLo RNA-Sequencing (RNA-seq) System following NuGen's standard protocol (M01406v2). For the first experiment (at P9) libraries were prepared with a starting amount of 1 ng and amplified in 14 PCR cycles. Libraries were profiled in a High Sensitivity DNA on a 2100 Bioanalyzer (Agilent technologies) and quantified using the ddPCR Library Quantification Kit for Illumina TruSeq in a QX200 Droplet Digital PCR system (BioRad). Details information on the NGS protocol are described in the SI.
Ovation solo rna sequencing rna seq system
Manufactured by Tecan
The Ovation SoLo RNA-Sequencing (RNA-seq) System is a lab equipment product designed for RNA sequencing. It provides a platform for analyzing gene expression and transcriptome profiling. The system enables the generation of high-quality RNA-seq data from small input samples.
Automatically generated - may contain errors
2 protocols using ovation solo rna sequencing rna seq system
1
RNA-Seq Library Preparation from Small Samples
2
RNA-seq analysis of NG2 sorted cells
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After NG2 sorting, cells were pelleted and RNA was extracted using RNeasy Micro Kit (QIAGEN) according to manufacturer's instructions. NGS library prep was performed with NuGen Ovation SoLo RNA-Sequencing (RNA-seq) System following NuGen`s standard protocol (M01406v2). For the first experiment (at P9) libraries were prepared with a starting amount of 1 ng and amplified in 14 PCR cycles. Libraries were profiled in a High Sensitivity DNA on a 2100 Bioanalyzer (Agilent technologies) and quantified using the ddPCR Library Quantification Kit for Illumina TruSeq in a QX200 Droplet Digital PCR system (BioRad). Details information on the NGS protocol are described in the SI.
After NG2 sorting, cells were pelleted and RNA was extracted using RNeasy Micro Kit (QIAGEN) according to manufacturer's instructions. NGS library prep was performed with NuGen Ovation SoLo RNA-Sequencing (RNA-seq) System following NuGen`s standard protocol (M01406v2). For the first experiment (at P9) libraries were prepared with a starting amount of 1 ng and amplified in 14 PCR cycles. Libraries were profiled in a High Sensitivity DNA on a 2100 Bioanalyzer (Agilent technologies) and quantified using the ddPCR Library Quantification Kit for Illumina TruSeq in a QX200 Droplet Digital PCR system (BioRad). Details information on the NGS protocol are described in the SI.
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