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2 protocols using hesperidin

1

Standardized Phytochemical Compound Acquisition

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Ferulic acid (99%) was purchased from Sigma Aldrich (St. Louis, MO, USA). Ascorbic acid and quercetin (≤100%) were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Caffeic acid (≥98%), curcumin (100%), hesperidin (90%), rutin (95.0–101.5%), and triiodothyronine (T3) were purchased from Nacalai Tesque, Inc. (Kyoto, Japan). Gallic acid (≥98%), chlorogenic acid (≥95%), ellagic acid (≥95%), sesamin (≥95%), epigallocatechin-3-gallate (≥96%), genistein (≥98%), daidzein (≥95%), luteolin (≥98%), theaflavin (≥98%), isoquercetin (98%), and morin (100%) were purchased from Cayman Chemical Company (Ann Arbor, MI, USA). Resveratrol (>99.0%), fisetin (>96.0%), isorhamnetin (>95.0%), and troxerutin (90%) were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Cyanidin chloride (99%) was purchased from Nagara Science Co. Ltd. (Gifu, Japan). Apigenin (≥98%) was purchased from LKT Laboratories, Inc. (St. Paul, MN, USA). Kaempferol (98%), myricetin (98%), and naringin (98%) were purchased from BDL Pharmatech Ltd. (Shanghai, China). Hyperoside (90–100%) was purchased from Toronto Research Chemicals Inc. (Toronto, ON, Canada). Neohesperidin (≥98%) was purchased from Chem-Impex International, Inc. (Wood Dale, IL, USA). All other chemicals were commercially available and had the highest possible purity.
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2

Quantitative Analysis of RVLE Extracts

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Materials OTC drugs and health foods containing RVLE extracts were purchased from pharmacies in Japan and from the Japanese market (Table 1). To obtain plural products with different lot numbers, one or two packages of each product were purchased several times during the period December 2013 to June 2015. Hesperidin and monoglucosyl Hesperidin were purchased from Nacalai Tesque (Kyoto, Japan) for peak identification. Quercetin 3-O-β-D-glucuronide (Q3GA), quercetin 3-O-β-D-glucoside (Q3G), kaempferol 3-O-β-D-glucoside (K3G), (-)-trans-ε-viniferin and pterostilbene were purchased from Funakoshi (Tokyo, Japan), and resveratrol was purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan) as the reference substances for calibrators (Fig. 1).
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