Quantification of aliphatic suberin was performed as described previously (Holbein et al., 2019 (link)). Eleven-day-old plants were treated with SDW (mock) or infected with A2-J or A2-O. At 4 DPI, root tips inoculated with nematodes were microscopically checked for infection, and more than 50 infected root tips were cut (approximately 3–5 mm from the tip) and collected for each treatment. To remove unbound lipids, samples were extracted in methanol for 24 h then in chloroform for 24 h, dried, and weighed. Samples were depolymerized and analyzed by gas chromatography-mass spectrometry (GC-MS) (Agilent 6890N-Agilent 5973N quadrupole mass-selective detector, Agilent Technologies, Germany) for monomer identification and for quantitative analysis based on an internal standard using an identical gas chromatography system coupled with a flame ionization detector as described previously (Franke et al., 2005 (link)).
Agilent 6890n agilent 5973n quadrupole mass selective detector
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Sourced in Germany
The Agilent 6890N-Agilent 5973N is a quadrupole mass-selective detector that functions as a core analytical instrument for identifying and quantifying chemical compounds. It combines a gas chromatograph (6890N) with a mass spectrometer (5973N) to provide comprehensive analytical capabilities.
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2 protocols using agilent 6890n agilent 5973n quadrupole mass selective detector
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Aliphatic Suberin Quantification in Nematode-Infected Root Tips
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Quantification of Aliphatic Suberin
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Quantification of aliphatic suberin was performed as described previously (Holbein et al., 2019) (link).
Eleven-day-old plants were treated with SDW (mock) or infected with A2-J or A2-O. At 4 DPI, root tips inoculated with nematodes were microscopically checked for infection, and more than 50 infected root tips were cut (approximately 3-5 mm from the tip) and collected for each treatment. To remove unbound lipids, samples were extracted in methanol for 24 h then in chloroform for 24 h, dried, and weighed. Samples were depolymerized and analyzed by GC-MS (Agilent 6890N-Agilent 5973N quadrupole mass-selective detector, Agilent Technologies, Germany) for monomer identification and for quantitative analysis based on an internal standard using an identical GC system coupled with a flame ionization detector as described previously (Franke et al., 2005) .
Eleven-day-old plants were treated with SDW (mock) or infected with A2-J or A2-O. At 4 DPI, root tips inoculated with nematodes were microscopically checked for infection, and more than 50 infected root tips were cut (approximately 3-5 mm from the tip) and collected for each treatment. To remove unbound lipids, samples were extracted in methanol for 24 h then in chloroform for 24 h, dried, and weighed. Samples were depolymerized and analyzed by GC-MS (Agilent 6890N-Agilent 5973N quadrupole mass-selective detector, Agilent Technologies, Germany) for monomer identification and for quantitative analysis based on an internal standard using an identical GC system coupled with a flame ionization detector as described previously (Franke et al., 2005) .
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