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Phiv luc zsgreen backbone

Manufactured by Addgene
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The PHIV-Luc-ZsGreen backbone is a plasmid vector that contains the Firefly luciferase (Luc) and ZsGreen reporter genes. It can be used for gene expression studies and reporter assays.

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Lab products found in correlation

Pcdna3 ova, by Addgene (2 mentions)

2 protocols using phiv luc zsgreen backbone

1

Lentiviral Constructs for Ovalbumin Expression

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
pLenti6-UbC-VKine-HA and pLenti6-UbC-VKine-Myc has been previously described (Hope et al., 2016 (link); McCulloch et al., 2009 (link)). Ovalbumin (OVA) amplicon was PCR amplified from pcDNA3-OVA (Addgene #64599) and cloned into pHIV-Luc-ZsGreen backbone (Addgene #39196). All lentiviral constructs were transformed into NEB 5-alpha competent cells (#C2987U) for propagation of plasmid DNA. All plasmids were prepped and purified using Macharey-Nagel NucleoBond Xtra Maxi kit (# 740414.50).
Papadas A., Deb G., Cicala A., Officer A., Hope C., Pagenkopf A., Flietner E., Morrow Z.T., Emmerich P., Wiesner J., Arauz G., Bansal V., Esbona K., Capitini C.M., Matkowskyj K.A., Deming D.A., Politi K., Abrams S.I., Harismendy O, & Asimakopoulos F. (2022). Stromal remodeling regulates dendritic cell abundance and activity in the tumor microenvironment. Cell reports, 40(7), 111201.
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2

Lentiviral Constructs for Ovalbumin Expression

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
pLenti6-UbC-VKine-HA and pLenti6-UbC-VKine-Myc has been previously described (Hope et al., 2016 (link); McCulloch et al., 2009 (link)). Ovalbumin (OVA) amplicon was PCR amplified from pcDNA3-OVA (Addgene #64599) and cloned into pHIV-Luc-ZsGreen backbone (Addgene #39196). All lentiviral constructs were transformed into NEB 5-alpha competent cells (#C2987U) for propagation of plasmid DNA. All plasmids were prepped and purified using Macharey-Nagel NucleoBond Xtra Maxi kit (# 740414.50).
Papadas A., Deb G., Cicala A., Officer A., Hope C., Pagenkopf A., Flietner E., Morrow Z.T., Emmerich P., Wiesner J., Arauz G., Bansal V., Esbona K., Capitini C.M., Matkowskyj K.A., Deming D.A., Politi K., Abrams S.I., Harismendy O, & Asimakopoulos F. (2022). Stromal remodeling regulates dendritic cell abundance and activity in the tumor microenvironment. Cell reports, 40(7), 111201.
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+ Expand

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