A total of 30 μg equivalent amount of protein per sample were loaded on 10% SDS–PAGE gel and transferred to nitrocellulose membranes for further processing. The membrane was blocked with 5% bovine serum albumin (BSA) for 1 hour at room temperature, followed by overnight incubation at 4°C with the primary antibodies [pEGFR T693 (1:300, cat. no. ab75980, Abcam, UK)]. Membranes were incubated with appropriate peroxidase-conjugate secondary antibodies (Santa Cruz, USA, 1:3000) and bands were visualized by the enhanced chemiluminescence (ECL) method (BioRad, USA).
Peroxidase conjugate secondary antibodies
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Peroxidase-conjugate secondary antibodies are laboratory reagents used in various immunoassays and detection techniques. They consist of secondary antibodies that are chemically conjugated to the enzyme peroxidase. The peroxidase enzyme can catalyze a color-producing reaction, enabling the detection and visualization of target analytes in the samples being analyzed.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using peroxidase conjugate secondary antibodies
1
Western Blot Analysis of pEGFR Phosphorylation
2
Western Blotting of Phospho-β-catenin
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A total of 30 μg equivalent amount of protein per sample were loaded on 10% SDS–PAGE gel and transferred to nitrocellulose membranes for further processing. The membrane was blocked with 5% bovine serum albumin (BSA) for 1 h at room temperature, followed by overnight incubation at 4 °C with the primary antibodies [phospho β-catenin Ser552 (CST #9566) (1:500), β-catenin (Abcam #ab32572) (1:500)]. Membranes were incubated with appropriate peroxidase-conjugate secondary antibodies (Santa Cruz, USA, 1:3000) and bands were visualized by the enhanced chemiluminescence (ECL) method (BioRad, USA).
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