Esquire 6000 esi ion trap

General synthetic procedures: Thin layer chromatography (TLC) analyses were performed on silica gel 60 F 254 precoated on aluminium plates (Merck) and the compounds were detected by staining with cerium (IV) sulfate (1 g)/ ammonium molybdate tetrahydrate (21 g)/sulfuric acid (30 mL) solution in water (0.47 L), or with anisaldehyde solution [anisaldehyde (25 mL), sulfuric acid (25 mL) and acetic acid (1 mL) in ethanol (450 mL)], followed by heating at over 200ºC. Size exclusion chromatography was performed using Sephadex LH-20 gel from GE Healthcare. Ion-exchange chromatography was carried out using Dowex 50WX2 Na + resin from Sigma-Aldrich. For ultrafiltration, Amicon Ultra-4 devices from Merck were employed. 1 H-and 13 C-NMR spectra were acquired on a Bruker Avance III-400 spectrometer. Bidimensional COSY and HSQC NMR experiments were carried out to assist in signal assignment. Electrospray mass spectra (ESI MS) of dendrons 3 and 4 were carried out with an Esquire 6000 ESI-Ion Trap from Bruker Daltonics, using a 10 mM diethylamine solution in a 1:1 MeOH/H2O mixture. To weigh compounds, such as the dendritic cores, in quantities lower than 1 mg, we prepared a stock solution and took the appropriate aliquot from it. After concentration and drying under high vacuum, we checked the weight by using a semi microbalance (5 decimal places).

General synthetic procedures: Thin layer chromatography (TLC) analyses were performed on silica gel 60 F254 precoated on aluminium plates (Merck) and the compounds were detected by staining with sulfuric acid/ethanol (1:9), with cerium (IV) sulfate (10 g)/phosphomolybdic acid (13 g)/sulfuric acid (60 mL) solution in water (1 L), or with anisaldehyde solution [anisaldehyde (25 mL) with sulfuric acid (25 mL), ethanol (450 mL) and acetic acid (1 mL)], followed by heating at over 200ºC. Column chromatography was carried out on silica gel 60 (0.2-0.5 mm, 0.2-0.063 mm or 0.040-0.015 mm; Merck). Optical rotations were determined with a Perkin-Elmer 341 polarimeter. 1 H-and 13 C-NMR spectra were acquired on Bruker DPX-300, Avance III-400 and DRX-500 spectrometers. Electrospray mass spectra (ESI MS) were carried out with an Esquire 6000 ESI-Ion Trap from Bruker Daltonics. High resolution mass spectra (HR MS) were carried out by CITIUS (Universidad de Sevilla). MALDI-TOF mass spectra (MALDI-TOF MS) were carried out with a microflex instrument from Bruker. Microwave-based reactions were performed using a Biotage Initiator Eight synthesizer in sealed reaction vessels.