Five micro molar frozen non-fixed sections of human skin (treated with 1 M NaCl) were blocked (1% BSA in PBS) and human sera in 1:4 to 1:320 dilutions were used as primary antibodies. Rabbit anti-human IgG-FITC (Abcam, Cambridge, Britain) was used as the secondary antibody. The skin sections were then mounted with glycerol/PBS (2:1, pH 9.0) and observed under a fluorescence microscope. A clear linear immunostaining on the BMZ was considered positive, while no fluorescence was considered negative.
Rabbit anti human igg fitc
Manufactured by Abcam
Rabbit anti-human IgG-FITC is a secondary antibody conjugated with the fluorescent dye fluorescein isothiocyanate (FITC). It is designed to detect and visualize human immunoglobulin G (IgG) in various immunological applications.
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Lab products found in correlation
2 protocols using rabbit anti human igg fitc
1
Immunostaining of Human Skin Sections
2
Indirect Immunofluorescence Assay for Skin Basement Membrane Zone
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SSS-IIF was performed as previously described (21 (link)). In brief, 5-µm frozen nonfixed sections of the human skin (treated with 1 M NaCl) were blocked (1% bovine serum albumin in phosphate-buffered saline) and incubated with patients’ serum at 1:4–1:320 dilution with rabbit anti-human IgG-FITC (Abcam), followed by routine indirect immunofluorescence microscopy. Clear linear immunostaining of the epidermal side of the basement membrane zone (BMZ) was considered positive, whereas no fluorescence was considered negative.
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