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Alexa fluor 568

Manufactured by Proteintech
Sourced in United States

Alexa Fluor 568 is a fluorescent dye produced by Proteintech. It is a bright, photostable dye with excitation and emission maxima of 578 nm and 603 nm, respectively. Alexa Fluor 568 can be used for a variety of fluorescence-based applications, including labeling of proteins, nucleic acids, and other biomolecules.

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2 protocols using alexa fluor 568

1

Immunofluorescence analysis of Cdc42 in cells

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Anti-CD9, CD63, Alix, calnexin, CD31, CD34, CD45, CD73, CD90 and CD105 antibodies were purchased from Abcam (Cambridge, UK). Anti-Ki-67, RhoA, Rac1, Cdc42 and β-actin antibodies were purchased from Cell Signaling Technology (Danvers, USA). Alexa Fluor 488 and Alexa Fluor 568 secondary antibodies were purchased from Proteintech (Rosemont, IL, USA). Goat anti-rabbit/anti-mouse IgG IRDyel 800cw secondary antibodies were purchased from Abbkine (Redlands, CA, USA). PKH-26 and PKH-67 kits were purchased from Sigma-Aldrich (St. Louis, MO, USA). Lipofectamine TM RNAiMAX, FM TM 4-64FX and ActinGreen TM 488 were purchased from Thermo Fisher (Eugene, Dregon, USA). SiCdc42, Cdc42-EGFP and Cdc42-mCherry fusion protein expression plasmids were purchased from GenePharma (Suzhou, China). Cdc42 inhibitor ML141 was purchased from MedChemExpress (Monmouth Junction, USA).
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2

Characterization of Cdc42 Regulators

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Anti-CD9, CD63, Alix, calnexin, and CD31 antibodies were purchased from Abcam (Cambridge, UK). Anti-Ki-67, RhoA, Rac1, Cdc42 and β-actin antibodies were purchased from Cell Signaling Technology (Danvers, USA). Alexa Fluor 488 and Alexa Fluor 568 secondary antibodies were purchased from Proteintech (Rosemont, IL, USA). Goat anti-rabbit/anti-mouse IgG IRDyel 800cw secondary antibodies were purchased from Abbkine (Redlands, CA, USA). PKH-67 kits were purchased from Sigma-Aldrich (St.
Louis, MO, USA). Lipofectamine TM RNAiMAX, FM TM 4-64FX and ActinGreen TM 488 were purchased from Thermo Fisher (Eugene, Dregon, USA). SiCdc42, Cdc42-EGFP and Cdc42-mCherry fusion protein expression plasmids were purchased from GenePharma (Suzhou, China). Cdc42 inhibitor ML141 was purchased from MedChemExpress (Monmouth Junction, USA).
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