Mdn 5s

Manufactured by Merck Group

Sourced in United States, Japan

The MDN-5S is a laboratory centrifuge designed for general-purpose applications. It features a compact and durable construction, with a maximum speed of 5,000 rpm and a maximum capacity of 5 x 15 mL tubes. The centrifuge is equipped with a brushless motor and an automatic rotor identification system for safe operation.

Automatically generated - may contain errors

Lab products found in correlation

5 protocols using mdn 5s

Gas, Liquid Chromatography and Mass Spectrometry Analysis of Lipids

Check if the same lab product or an alternative is used in the 5 most similar protocols

The GC analysis was carried out on a Shimadzu GC-2010 chromatography (Kyoto, Japan) with a flame ionization detector on a SUPELCOWAX 10 (Supelco, Bellefonte, PA, USA) capillary column (30 m × 0.25 mm × 0.25 μm). Carrier gas was He at 30 cm/s. The GC–MS analysis was performed with a Shimadzu CMS-QP5050A instrument (Kyoto, Japan) (electron impact at 70 eV) with a MDN-5s (Supelco, Bellefonte, PA, USA) capillary column (30 m × 0.25 mm ID). Carrier gas was He at 30 cm/s.
The HPLC–HRMS analysis of polar lipids was performed with a Shimadzu Prominence liquid chromatograph equipped with two LC-20AD pump units, a high pressure gradient forming module, CTO-20A column oven, SIL-20A auto sampler, CBM-20A communications bus module, DGU-20A3 degasser, and a Shim-Pack diol column (50 mm × 4.6 mm ID, 5 μm particle size) (Shimadzu, Kyoto, Japan). Lipids were detected by a high resolution tandem ion trap–time of flight mass spectrometry with a Shimadzu LCMS-IT-TOF instrument (Kyoto, Japan) operating both at positive and negative ion mode during each analysis at electrospray ionization (ESI) conditions. Ion source temperature was 200 °C, the range of detection was m/z 200–1600, and potential in the ion source was −3.5 and 4.5 kV for negative and positive modes, respectively. The drying gas (N₂) pressure was 200 kPa. The nebulizer gas (N₂) flow was 1.5 L/min.

Tran Q.T., Le T.T., Pham M.Q., Do T.L., Vu M.H., Nguyen D.C., Bach L.G., Bui L.M, & Pham Q.L. (2019). Fatty Acid, Lipid Classes and Phospholipid Molecular Species Composition of the Marine Clam Meretrix lyrata (Sowerby 1851) from Cua Lo Beach, Nghe An Province, Vietnam. Molecules, 24(5), 895.

+ Open protocol

+ Expand

Analyzing Compounds via GC-MS and NMR

Check if the same lab product or an alternative is used in the 5 most similar protocols

Products were analysed as Me/TMS derivatives by GC–MS. The GC–MS analyses were performed using a Shimadzu QP5050A mass spectrometer connected to a Shimadzu GC-17A gas chromatograph equipped with a Supelco MDN-5S (5% phenyl, 95% methylpolysiloxane)-fused capillary column (length, 30 m; ID 0.25 mm; film thickness, 0.25 μm). Helium at a flow rate of 30 cm/s was used as the carrier gas. Injections were made in split mode using an initial column temperature of 120 °C and injector temperature 230 °C. The column temperature was raised at 10 °C/min until 240 °C. The electron impact ionization (70 eV) was used. Most GC–MS analyses were carried out in full-scan mode. The NMR ¹H, ¹H-¹H-COSY, ¹H-¹³C-HSQC, ¹H-¹³C-HMBC, ¹H-¹H-NOESY and ¹H-¹H-TOCSY spectra ([²H₁₄]n-hexane) were recorded on a Bruker Avance III 600 spectrometer at 253 K.

Grechkin A.N., Lantsova N.V., Mukhtarova L.S., Khairutdinov B.I., Gorina S.S., Iljina T.M, & Toporkova Y.Y. (2023). Distinct Mechanistic Behaviour of Tomato CYP74C3 and Maize CYP74A19 Allene Oxide Synthases: Insights from Trapping Experiments and Allene Oxide Isolation. International Journal of Molecular Sciences, 24(3), 2230.

+ Open protocol

+ Expand

GC-MS Analysis of Volatile Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

A Shimadzu GC-MSQP2010 fitted with a AOC-20i injector and FID and MS detectors (Shimadzu, Tokyo, Japan) was used for the GC-MS analysis. The GC was run with a nonpolar Supelco MDN-5S fused silica capillary column (30 m × 0.25 mm ID, 0.25 µm film thickness) commonly used for the analysis of VOCs. The injection volume (EO dissolved in chromatography grade hexane) was 1 μL. Helium was used as the carrier gas at a constant flow of 1 mL/min. The column temperature was set at 50 °C for 30 s, then increased from 50–150 °C at 4 °C/min, from 150–175 °C at 1.5 °C/min and from 175–300 °C at 20 °C/min for a total analysis time of 58.42 min. The injector temperature was set to 250 °C and the injection was accomplished with a split ratio of 1/50 throughout the entire run. The MS was operated in the electron impact mode at 70 eV, with a scan range of 40–400 m/z. The temperatures were set to 200 °C for the ion trap, 50 °C for the manifold and 305 °C for the transfer line.
A Varian 450-GC fitted with an MS240 iontrap MS and a Combipal autosampler (Varian Instruments, Sunnyvale, CA, USA) and run with a nonpolar Varian FactorFour VF-5ms column (30 m × 0.25 mm ID, 0.25 μm film) commonly used for the analysis of VOCs was used under the same conditions to assist in compound identification.

Rodrigues A.M., Eparvier V., Odonne G., Amusant N., Stien D, & Houël E. (2019). The antifungal potential of (Z)-ligustilide and the protective effect of eugenol demonstrated by a chemometric approach. Scientific Reports, 9, 8729.

+ Open protocol

+ Expand

GC-MS Analysis of Fatty Acid Derivatives

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fatty acid methyl esters (FAME) were obtained by treating the lipids with 2% H₂SO₄/MeOH at 80°C for 2 h in a screw-caped vial under argon, extracted with hexane and purified by preparative TLC developed in benzene. 4,4-Dimethyloxazoline (DMOX) derivatives of FAs were prepared according to [53 (link)]. A gas chromatography analysis of FAME was conducted on a GC-2010 chromatograph (Shimadzu, Kyoto, Japan) with a flame ionization detector. A SUPELCOWAX 10 (Supelco, Bellefonte, PA) capillary column (30 m × 0.25 mm i.d.) was used at 210°C. The injector and detector temperatures were 240°C. Helium was used as the carrier gas at a linear velocity of 30 cm/s. The identification of FAs was confirmed by gas chromatography–mass spectrometry (GC–MS) of their methyl esters and DMOX derivatives using a GCMS-2010 Ultra instrument (Shimadzu, Kyoto, Japan) (electron impact at 70 eV) and a MDN-5s (Supelco, Bellefonte, PA) capillary column (30 m × 0.25 mm ID). The carrier gas was He at 30 cm/s. The GC–MS analysis of FAME was performed at 160°C with a 2°C/min ramp to 240°C that was held for 20 min. The injector and detector temperatures were 250°C. GC–MS of DMOX derivatives was performed at 210°C with a 3°C/min ramp to 270°C that was held for 40 min. The injector and detector temperatures were 270°C. Spectra were compared with the NIST library and FA mass spectra archive [54 ].

Imbs A.B., Dang L.P, & Nguyen K.B. (2019). Comparative lipidomic analysis of phospholipids of hydrocorals and corals from tropical and cold-water regions. PLoS ONE, 14(4), e0215759.

+ Open protocol

+ Expand

Analysis of M. quinquenervia Essential Oils

Check if the same lab product or an alternative is used in the 5 most similar protocols

The essential oils of M. quinquenervia were analyzed by gas chromatography with flame ionization detector (GC-FID) using a Shimadzu GC-2014 gas chromatograph. The data obtained was on a 5% diphenyl-/95% dimethylpolysiloxane fused silica capillary column (30m x 0,25mm; film thickness 0,25μm), (MDN-5S, Supelco). The GC integrations were performed with a LabSolutions, Shimadzu GC Solution, Chromatography Data System, software version 2.3. The operating conditions used were: carrier gas N2, flow 1,0mL/min; oven temperature program: (60 to 280) °C at 3 °C/min, 280 °C (2 min); sample injection port temperature 250 °C; detector temperature 280 °C; split 1:60.

Casanova J., & Chaverri C. (2021). Chemical composition of essential oils of the tree Melaleuca quinquenervia (Myrtaceae) cultivated in Costa Rica. UNED Research Journal, 13(1), 10-10.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!