Lung cancer cell lines H1435, H522 and LL/2 were purchased from American Type Culture Collection. CMT167 was purchased from Sigma. H1435, H522,CMT167 and LL/2 were cultured in RPMI medium supplemented with 10%FBS, streptomycin (100mg/ml) and penicillin (100units/ml). All cells were grown at 37 °C in a 5% CO2 atmosphere. All cells were tested negative of Mycoplasma by PCR (ATCC Universal Mycoplasma Detection Kit), and they were used between passages 10 and 35. All cell lines were obtained between 2019 and 2021, and they were authenticated by qRT-PCR analysis for the expression of 20 signature genes.
H1435
Manufactured by American Type Culture Collection
Sourced in United States
The H1435 is a piece of laboratory equipment designed for cell culture applications. It functions as an incubator, providing a controlled environment for the growth and maintenance of cell lines. The H1435 maintains specific temperature, humidity, and carbon dioxide levels to support optimal cell culture conditions.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (4 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Beas 2b, by American Type Culture Collection (3 mentions)
H2030, by American Type Culture Collection (3 mentions)
H1395, by American Type Culture Collection (2 mentions)
Penicillin, by Merck Group (2 mentions)
Streptomycin, by Merck Group (2 mentions)
H1975, by American Type Culture Collection (2 mentions)
Penicillin, by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
L glutamine, by Thermo Fisher Scientific (2 mentions)
Sirna, by Thermo Fisher Scientific (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Cmt167, by Merck Group (1 mentions)
Universal mycoplasma detection kit, by American Type Culture Collection (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
H1819, by American Type Culture Collection (1 mentions)
Hcc15, by American Type Culture Collection (1 mentions)
Hek293t, by American Type Culture Collection (1 mentions)
H1299, by American Type Culture Collection (1 mentions)
11 protocols using h1435
1
Lung Cancer Cell Line Cultivation
2
Lung and Squamous Cancer Cell Lines
3
Cell Culture of Human Cancer Lines
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Human cell lines used in this study included the lung adenocarcinoma cell line H1435 (American Type Culture Collection [ATCC] CRL-5870), the embryonic kidney cell line HEK-293T (ATCC CRL-3216), and the lung adenocarcinoma cell line CL1-5 (provided by Dr. Cheng-Wen Wu [52 (link)]). Cells were cultured in Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12; for H1435 and CL1-5 cells) or high-glucose DMEM media (HEK-293T cells) at 37°C in a humidified atmosphere containing 5% CO2. Media were supplemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine, and 1% penicillin/streptomycin.
4
BALB/c Mice for Lung Cancer
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Male 8 weeks BALB/c nu/nu mice (N = 30; randomly divided into three groups) were obtained from the National Laboratory of Animal Breeding and Research Center (Taipei, Taiwan) and housed according to the protocols established by the Animal Center of the Kaohsiung Medical University (Kaohsiung, Taiwan). Human lung cancer cell lines (A549, H358, H441, H1299, H1435, and H1437), and human diploid lung fibroblast W138 were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA) and maintained according to their protocols. The study was conducted with approval (IACUC‐104181) from the ethics committee of Kaohsiung Medical University.
5
Silencing NFASC in Lung Cancer Cell Lines
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Lung cancer cell lines H838, H460, H23, and H1435 were obtained from American Type Culture Collection (Rockville, MD) and authenticated in 2011 using DNA fingerprinting (Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany). Cells were maintained in RPMI‐1640 medium (ThermoFisher Scientific) with 10% FCS (ThermoFisher Scientific) and penicillin/streptomycin (Biowest SAS, Nuaillé, France) in 5% CO2 at 37°C. Cells were passaged every 2nd or 3rd day. RNA silencing experiments were conducted in penicillin/streptomycin free medium in 6‐well plates. The cells were seeded at the following concentrations: H838, 2.0E5 cells/well; H460, 3.0E5 cells/well; H23, 6.0E5 cells/well; and H1435, 1.5E6 cells/well. siRNA targeting human NFASC and non‐target control were purchased from Applied Biosystems (ThermoFisher Scientific). Transfections were performed 24 h after seeding using 10 nM siRNA and Lipofectamin RNAiMAX reagent (Invitrogen, ThermoFisher Scientific) according to manufacturer's instructions. After 48 h the cells were used for functional analysis or harvested for analysis of RNA. Protein was extracted 72 h after transfection.
6
Lung Cancer Cell Line Cultivation
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Human lung cancer cell lines (A-549, H-23, H1573, H-1373, H-1734, H-2347, H-2444, H-1650, H-522, Calu-3, H-1395, H-1435, H-1838, H-2228, H-2286, A-427) were purchased from American Type Culture Collection (ATCC). A-549 and Calu-3 cells were maintained and passaged in Dulbecco’s modified Eagle medium (DMEM) (Gibco, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Gibco, Thermo Fisher Scientific) and 1% antibiotic-antimicotic solution (Gibco, Thermo Fisher Scientific) in a humidified incubator at 37°C and 5% CO2. H-23, H1573, H-1373, H-1734, H-2347, H-2444, H-1650, H-522, H-1395, H-1838, H-2228, and A-427 cells were maintained and passaged in Roswell Park Memorial Institute–1640 medium (Gibco, Thermo Fisher Scientific) supplemented with 10% FBS (Gibco, Thermo Fisher Scientific) and 1% antibiotic-antimicotic solution (Gibco, Thermo Fisher Scientific) in a humidified incubator at 37°C and 5% CO2. H-1435 and H-2286 cells were maintained and passaged in DMEM/nutrient mixture F12 (Gibco, Thermo Fisher Scientific) supplemented with 10% FBS and 1% antibiotic-antimicotic solution in a humidified incubator at 37°C and 5% CO2. The summary of signature genetic changes of the cell lines is listed in Supplemental Table 3 .
7
HeLaS3 miR-21 EGFPB Biosensor Generation
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The miR-21 EGFP based biosensor (HeLaS3 miR-21 EGFPB) harboring a reporter for miRNA activity was generated as previously described. In brief, HeLaS3 cells were transfected with pcDNA/TO/EGFPmiR21 (Addgene, Cambridge, MA) using Lipofectamine 2000 transfection reagent and Zeocin-resistant cells were harvested for storage of cell stocks at −170°C. A549, 451Lu, A375, H460, H838, H1435, H2030, HCC1954, HEK293, HeLa, HeLaS3, MDA-MB-231, RPE, SK-MEL-5, SK-MEL-28, SK-MEL-94, SK-MEL-100, SK-MES-1 cell lines were purchased from American Type Culture Collection (ATCC, Manassas, VA). JIMT-1 cell line was purchased from AddexBio (San Diego, CA). LK-2 cell line was purchased from RIKEN BioResource Center (Japan). The isogenic DICER1 knockout pairs in DLD1, HCT116, and RKO cell lines were purchased from Horizon Discovery (Cambridge, United Kingdom). All cell lines were cultured at 37°C with 5% CO2-95% air; in addition, supplies were from Thermo Fisher Scientific (Waltham, MA) and Sigma-Aldrich (St Louis, MO).
8
Cell Culture Conditions for LUAD Lines
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LUAD cell lines (A549, H1975, H2030, H1435) and a normal human bronchial epithelial cell (BEAS-2B) were provided by the American Type Culture Collection (ATCC; Gaithersburg, MD, USA). Briefly, all cancer cells applied in this study were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Waltham, MA, USA) containing 10% fetal bovine serum (FBS; Gibco, Waltham, MA, USA), 100 U/ml penicillin (Sigma-Aldrich, USA), and 100 mg/ml streptomycin (Sigma-Aldrich, USA) at 37 °C with 5% CO2. BEAS-2B cell lines were cultured in Clonetics™ media.
9
Acquisition and Culture of Lung Adenocarcinoma Samples
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Twenty pairs of LUAD specimens and adjacent non-tumor tissues were acquired from patients who did not receive any therapy before undergoing operation in Sir Run Run Hospital of Nanjing Medical University. Tissue specimens were obtained from patients who signed informed consent. Research was performed in accordance with the Declaration of Helsinki and approval was obtained from the Ethics Committee of the Sir Run Run Hospital of Nanjing Medical University. Immediately after the operation, tissue samples were frozen in liquid nitrogen and maintained at -80 °C.
Cell culture LUAD cell lines (A549, H1975, H2030, H1435) and a normal human bronchial epithelial cell (BEAS-2B) were provided by the American Type Culture Collection (ATCC; Gaithersburg, MD, USA). Brie y, all cells applied in this study were maintained in Dulbecco's modi ed Eagle's medium (DMEM; Gibco, Waltham, MA, USA) containing 10% fetal bovine serum (FBS; Gibco, Waltham, MA, USA), 100 U/ml penicillin (Sigma-Aldrich, USA), and 100 mg/ml streptomycin (Sigma-Aldrich, USA) at 37 °C with 5% CO 2 .
Cell culture LUAD cell lines (A549, H1975, H2030, H1435) and a normal human bronchial epithelial cell (BEAS-2B) were provided by the American Type Culture Collection (ATCC; Gaithersburg, MD, USA). Brie y, all cells applied in this study were maintained in Dulbecco's modi ed Eagle's medium (DMEM; Gibco, Waltham, MA, USA) containing 10% fetal bovine serum (FBS; Gibco, Waltham, MA, USA), 100 U/ml penicillin (Sigma-Aldrich, USA), and 100 mg/ml streptomycin (Sigma-Aldrich, USA) at 37 °C with 5% CO 2 .
10
Culturing Lung Adenocarcinoma Cell Lines
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The lung adenocarcinoma lines, A549, H23 and H1435, were purchased from the American Type Culture Collection, (Manassas, Virginia, USA) and respectively cultured in DMEM, RPMI and DMEM mixed with F12K (1:1) medium containing 10% fetal bovine serum, l -glutamine (2 mM), streptomycin (100 μg/mL) and penicillin (100 IU/mL) (all from Gibco-Invitrogen Corp., Carlsbad, CA, USA). Cells were incubated at 37 °C in a humidified atmosphere containing 5% CO2.
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