Electrochemiluminescence elecsys insulin

Blood samples were taken from all subjects after a 12-h fasting period to measure serum concentrations of glucose, insulin, SUA and creatinine. Commercial kits, normally used for routine examinations of patients, were employed for all analyses. In detail: enzymatic method with hexokinase Glucose HK Gen.3 Cobas Roche, for glucose assay; immuno Assay in ElectroChemiLuminescence Elecsys Insulin Cobas Roche for insulin assay; colorimetric enzymatic test Uric Acid 2 Cobas Roche for uric acid assay; colorimetric kinetic test based on the Jaffé method Creatinine Jaffé Gen.2 Cobas Roche for creatinine assay. HOMA-index was calculated by dividing the product of serum insulin (μU/ml) and serum glucose (mmol/L) by 22.5 (22 (link)). Glomerular filtration rate was estimated (eGFR) by means of the Schwartz formula using serum creatinine and height measurements and a k constant of 0.55 (23 (link)).

Blood samples were taken from all subjects after a 12-h fasting period in order to measure serum concentrations of total cholesterol, high density lipoprotein (HDL), Triglycerides, glucose, insulin, uric acid and creatinine. Commercial kits, normally used for routine examinations of patients, were employed for all analyses. In detail: enzymatic colorimetric test Cholesterol Gen.2 Cobas Roche, for total cholesterol assay; colorimetric enzymatic test in homogeneous phase HDL-Cholesterol Gen.4 Cobas Roche, for HDL cholesterol; enzymatic colorimetric test Triglycerides Cobas Roche, for Triglycerides assay; enzymatic method with hexokinase Glucose HK Gen.3 Cobas Roche, for glucose assay; immunoassay in ElectroChemiLuminescence Elecsys Insulin Cobas Roche, for insulin assay; colorimetric enzymatic test Uric Acid 2 Cobas Roche, for uric acid assay; and the colorimetric kinetic test based on the Jaffé method, Creatinine Jaffé Gen.2 Cobas Roche, for creatinine assay. LDL cholesterol was calculated using Friedewald’s formula, LDL cholesterol = total cholesterol − [HDL cholesterol + (triglyceridemia/5)]. HOMA index was calculated by dividing the product of serum insulin (µU/mL) and serum glucose (mmol/L) by 22.5 [17 (link)]. Glomerular filtration rate was estimated (eGFR) by means of the Schwartz formula using serum creatinine and height measurements and a k constant of 0.55 [18 (link)].