Dulbecco’s modified Eagle’s medium/Nutrient Mixture F-12 Ham’s (DMEM/F12) and fetal bovine serum (FBS) were purchased from HyClone (Logan, UT, USA). B-27 (50X) Serum-Free Supplement was from Gibco (Grand Island, NY, USA). Epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) were obtained from Peprotech (Rocky Hill, NJ, USA). Leukemia inhibitory factor (LIF) was obtained from ProSpec-Tany TechnoGene Ltd. (Rehovot, Israel). The CD133 cell isolation kit [magnetic cell sorting (MACS) method] was purchased from Miltenyi Biotec GmbH (Bergisch Gladbach, Germany). Antibodies to nestin, glial fibrillary acidic protein (GFAP) and tubulin-β were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Cell Counting kit-8 (CCK-8) was obtained from Dojindo Molecular Technologies, Inc. (Kumamoto, Japan). Cell Cycle and Apoptosis Analysis kits and trypsin were obtained from Beyotime Institute of Biotechnology (Shanghai, China). Lentivirus was purchased from Shanghai GeneChem Co., Ltd. (Shanghai, China).
Leukemia inhibitory factor
Manufactured by ProSpec
Sourced in Israel
Leukemia inhibitory factor (LIF) is a cytokine that plays a crucial role in stem cell biology. LIF is involved in the maintenance of pluripotency and self-renewal of embryonic stem cells. It is an essential component in the culture of embryonic and induced pluripotent stem cells.
Automatically generated - may contain errors
Lab products found in correlation
Basic fibroblast growth factor (bfgf), by Thermo Fisher Scientific (2 mentions)
Cell cycle and apoptosis analysis kit, by Beyotime (1 mentions)
Cd133 cell isolation kit, by Miltenyi Biotec (1 mentions)
Epidermal growth factor (egf), by Thermo Fisher Scientific (1 mentions)
Lentivirus, by Genechem (1 mentions)
Trypsin, by Beyotime (1 mentions)
Heparin, by Merck Group (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Hepes, by Biosera (1 mentions)
Dmem f12 medium, by Biosera (1 mentions)
T 25 ultra low attachment flask, by Corning (1 mentions)
Collagenase type 1, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Biosera (1 mentions)
Streptomycin, by Biosera (1 mentions)
Amphotericin b, by Biosera (1 mentions)
2 protocols using leukemia inhibitory factor
1
Isolation and Characterization of Neural Stem Cells
2
Gastric Cancer Tumor Sphere Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Fresh tumor specimens (0.3-4 mL) obtained from GC patients (Table 1 ) were disaggregated into single cell suspension in DMEM/F12 medium (Biosera, France) containing collagenase type I (Gibco, USD)
(300 U.mL-1), penicillin (Biosera) (500 U.mL-1), streptomycin (Biosera) (500 mg.mL-1), and amphotericin B (Biosera) (1.25 mg.mL-1) at 37 °C for 2 hr. The cells (1 × 105 cell.mL-1) were cultivated in epidermal growth factor (EGF) 20 ng.mL-1 (Gibco), basic fibroblast growth factor (bFGF) 10 ng.mL-1 (Gibco), leukemia inhibitory factor (LIF) 10 ng.mL-1 (ProSpec, Israel), heparin 4 μg.mL-1 (Sigma-Aldrich, Germany), B-27 supplement 2% (Gibco), penicillin 100 U.mL-1, and streptomycin 100 μg.mL-1, and HEPES 8 mM (Biosera) in DMEM/F12 medium in T-25 ultra-low attachment flask (Corning, USA) at 37 °C for 1-2 months in a humidified 5% CO2 incubator to generate spheres.
(300 U.mL-1), penicillin (Biosera) (500 U.mL-1), streptomycin (Biosera) (500 mg.mL-1), and amphotericin B (Biosera) (1.25 mg.mL-1) at 37 °C for 2 hr. The cells (1 × 105 cell.mL-1) were cultivated in epidermal growth factor (EGF) 20 ng.mL-1 (Gibco), basic fibroblast growth factor (bFGF) 10 ng.mL-1 (Gibco), leukemia inhibitory factor (LIF) 10 ng.mL-1 (ProSpec, Israel), heparin 4 μg.mL-1 (Sigma-Aldrich, Germany), B-27 supplement 2% (Gibco), penicillin 100 U.mL-1, and streptomycin 100 μg.mL-1, and HEPES 8 mM (Biosera) in DMEM/F12 medium in T-25 ultra-low attachment flask (Corning, USA) at 37 °C for 1-2 months in a humidified 5% CO2 incubator to generate spheres.
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