Inveon acquisition workplace 1

Animals were fasted for 6 h prior to the FDG injection and doxycycline treatment was interrupted due to the necessary sucrose addition to the drinking water. ~ 13 MBq FDG in a max. volume of 100 μl were injected i.v. into the tail vein under 1.5% isoflurane narcosis evaporated in oxygen at a flow rate of 0.5 l/min (Abbott, Wiesbaden, Germany) and the animals were kept under anesthesia for 55 min. post-injection in a heated box. Blood glucose and body weight measurements were performed immediately before FDG injection. Subsequently animals were placed on a carbon bed and scanned for 10 min in an Inveon small animal PET scanner (Siemens Preclinical Solutions, Knoxville, TN, USA). Body temperature was maintained at 37 °C by a heating pad and a rectal temperature sensor. Image reconstruction was performed using Inveon Acquisition Workplace 1.5.0.28 (Siemens Preclinical Solutions, Knoxville, TN, USA) with an iterative ordered-subset expectation maximization algorithm (OSEM2D) with four iterations. No attenuation and scatter correction was applied, according to our standard protocol for PET imaging with mice. The reconstructed voxel size was 0.776 × 0.776 × 0.796 mm. Images were analyzed in Inveon Research Workplace (Siemens Preclinical Solutions, Knoxville, TN, USA).