Log In Sign up
The largest database of trusted experimental protocols

Klebsiella pneumoniae atcc 700603

Manufactured by American Type Culture Collection
Visit Supplier
Sourced in Germany, United States

Klebsiella pneumoniae ATCC 700603 is a bacterial strain from the American Type Culture Collection. It is a well-characterized reference strain for research and microbial identification purposes.

Automatically generated - may contain errors

Lab products found in correlation

Escherichia coli atcc 25922, by American Type Culture Collection (4 mentions) Pseudomonas aeruginosa atcc 27853, by American Type Culture Collection (4 mentions) E coli atcc 25922, by American Type Culture Collection (3 mentions) Enterococcus faecalis atcc 29212, by American Type Culture Collection (2 mentions) Staphylococcus aureus atcc 25923, by American Type Culture Collection (2 mentions) Bacillus subtilis atcc 6633, by American Type Culture Collection (1 mentions) Silver acetate, by Merck Group (1 mentions) 6 hydroxy 2 5 7 8 tetramethylchroman 2 carboxylic acid trolox, by Thermo Fisher Scientific (1 mentions) 2 2 diphenyl 1 picrylhydrazyl (dpph), by Thermo Fisher Scientific (1 mentions) 2 2 azino bis 3 ethylbenzothiazoline 6 sulphonic acid abts, by Merck Group (1 mentions) P aeruginosa atcc 27853, by American Type Culture Collection (1 mentions) Potassium bromide kbr, by Merck Group (1 mentions) Methanol, by Merck Group (1 mentions) Folin ciocalteu reagent, by Merck Group (1 mentions) Silver nitrate, by Merck Group (1 mentions) S aureus atcc, by American Type Culture Collection (1 mentions) Ferrous chloride, by Merck Group (1 mentions) Multiskan go microplate spectrophotometer, by Thermo Fisher Scientific (1 mentions) Candida albicans atcc 10231, by American Type Culture Collection (1 mentions) Pseudomonas aeruginosa atcc 9027, by American Type Culture Collection (1 mentions)

9 protocols using klebsiella pneumoniae atcc 700603

1

Antibacterial Evaluation of Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols
The antibacterial activity of each compound was evaluated using six bacterial strains: three strains of Gram-positive (Staphylococcus aureus ATCC 292313, Bacillus subtilis ATCC 6633, and Enterococcus faecalis ATCC 29212) and three strains of Gram-negative (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, and Klebsiella pneumoniae ATCC 700603) bacteria. The bacterial strains were purchased from American Type Culture Collection (ATCC) (Manassas, VA, USA).
Kania A., Tejchman W., Pawlak A.M., Mokrzyński K., Różanowski B., Musielak B.M, & Greczek-Stachura M. (2022). Preliminary Studies of Antimicrobial Activity of New Synthesized Hybrids of 2-Thiohydantoin and 2-Quinolone Derivatives Activated with Blue Light. Molecules, 27(3), 1069.
+ Open protocol
+ Expand
2

Antimicrobial Potential of Beech Bark Extract

Check if the same lab product or an alternative is used in the 5 most similar protocols
The beech bark used for experimental research comes as waste from a wood processing company from Vatra Dornei, Romania. Before the extraction process, the bark was dried under normal temperature and aeration conditions (room temperature), and ground using a grinding mill type VEB NOSSENER MASCHINENBAU 1980. After grinding, the plant product was sieved through sieve 5.
Silver nitrate (AgNO3, M—169.87 g/mol, d—4.35 g/cm³), silver acetate (AgC2H3O2, M—166.91 g/mol, d—3.26 g/cm³), Folin–Ciocalteu reagent, potassium bromide (KBr), and methanol were purchased from Merck Company (Darmstadt, Germany), 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid)-(ABTS) was from Sigma-Aldrich (Steinheim, Germany), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) were obtained from Alfa-Aesar (Karlsruhe, Germany).
The following bacterial reference strains, stored at −70 °C in the Microbiology Laboratory from UMFST Târgu Mureș, were used: S. aureus ATCC (American Type Culture Collection) 25923, methicillin-resistant S. aureus (MRSA) ATCC 43300, E. coli ATCC 25922, Klebsiella pneumoniae ATCC 700603, P. aeruginosa ATCC 27853.
Tanase C., Berta L., Coman N.A., Roșca I., Man A., Toma F., Mocan A., Jakab-Farkas L., Biró D, & Mare A. (2019). Investigation of In Vitro Antioxidant and Antibacterial Potential of Silver Nanoparticles Obtained by Biosynthesis Using Beech Bark Extract. Antioxidants, 8(10), 459.
+ Open protocol
+ Expand
3

Antimicrobial Assessment of Metal Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols
LB media, zinc gluconate (TCI chemicals Pvt. Ltd, India), ferrous chloride (Sigma-Aldrich) were procured from the respective commercial sources. Staphylococcus aureus-ATCC 25923;
Escherichia coli-ATCC 25922; Pseudomonas aeruginosa-ATCC 27853 and Klebsiella pneumoniae-ATCC 700603 were purchased from ATCC, USA. Clinical specimens were obtained from PGIMER, Chandigarh after receiving the requisite ethics committee approval.
Kaushik S., Thomas J., Panwar V., Murugesan P., Chopra V., Salaria N., Singh R., Roy H.S., Kumar R., Gautam V., & Ghosh D. (2021). A Drug-free, Self-destruction Strategy to Combat Bacterial Infections by Using the Intrinsically formed Magnetic Nanoparticles in Bacterial Pathogens.
+ Open protocol
+ Expand
4

Antimicrobial Susceptibility Testing Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The Acinetobacter baumannii ATCC BAA-1605, Candida albicans ATCC 10231, Enterococcus faecium ATCC 700221, Klebsiella aerogenes ATCC 13048 (previously known as Enterobacter aerogenes), Klebsiella pneumoniae ATCC 700603, Pseudomonas aeruginosa ATCC 9027, Staphylococcus aureus ATCC 25923 and MRSA ATCC 33591, were acquired from the American Type Culture Collection (ATCC). Before running the tests, beads with cryo-protected microorganisms were transferred into fresh MHB (BioMaxima, Lublin, Poland) for bacteria or RPMI-1640 for fungi and incubated for 24 h at 37 °C. Then, the culture was seeded on the Mueller-Hinton agar (MHA) plates (BioMaxima, Lublin, Poland) for bacteria or Sabouraud dextrose agar (SDA) plates (BioMaxima, Lublin, Poland) for fungi and incubated as just mentioned. Cell densities for all assays were adjusted spectrophotometrically (Multiskan™ GO Microplate Spectrophotometer, Thermo Fisher Scientific, Vantaa, Finland) at 600 nm for bacteria and at 530 nm for fungi.
Neubauer D., Jaśkiewicz M., Bauer M., Gołacki K, & Kamysz W. (2020). Ultrashort Cationic Lipopeptides–Effect of N-Terminal Amino Acid and Fatty Acid Type on Antimicrobial Activity and Hemolysis. Molecules, 25(2), 257.
+ Open protocol
+ Expand
5

Bacterial Strain Acquisition and Storage Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Klebsiella oxytoca (ATCC 49131) and Klebsiella pneumoniae (ATCC 700603) were purchased from the American Type Culture Collection (ATCC).
Staphylococcus aureus (CIP 4.83), methicillin-resistant Staphylococcus aureus MRSA (CIP 107422), Escherichia coli (CIP 53.126), Acinetobacter baumannii (CIP 70.34), Pseudomonas aeruginosa (CIP 82.118), Enterococcus cloacae (CIP 103475), and Enterococcus faecium (CIP 102379) were purchased from “Collection de l’Institut Pasteur” (CIP, Pasteur Institute Collection).
Master stocks of bacterial strains were stored in a −80 °C freezer.
Johansen B., Duval R.E, & Sergere J.C. (2022). First Evidence of a Combination of Terpinen-4-ol and α-Terpineol as a Promising Tool against ESKAPE Pathogens. Molecules, 27(21), 7472.
+ Open protocol
+ Expand
6

Antimicrobial Susceptibility Testing Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The bacterial strains E. coli ATCC 25922, Pseudomonas aeruginosa ATCC 29212, and Klebsiella pneumoniae ATCC 700603 were obtained from ATCC (American Type Culture Collection, Manassas, VA). Metallo-β-lactamase (NDM1) clinical isolates, K. pneumoniae, and Acinetobacter baumannii, obtained from hospital settings, were also included in the study. Cation-adjusted Mueller-Hinton broth (MHB; Becton-Dickinson, Cockeysville, MD) was used for minimum inhibitory concentration (MIC) determination. Mueller-Hinton agar (MHA; Becton-Dickinson) was used for minimum bactericidal concentration (MBC) studies. Trypticase soy agar (TSA; Becton-Dickinson) was used for culturing of bacteria and colony counts.
Sharma R., Lambu M.R., Jamwal U., Rani C., Chib R., Wazir P., Mukherjee D., Chaubey A, & Khan I.A. (2016). Escherichia coli N-Acetylglucosamine-1-Phosphate-Uridyltransferase/Glucosamine-1-Phosphate-Acetyltransferase (GlmU) Inhibitory Activity of Terreic Acid Isolated from Aspergillus terreus. Journal of biomolecular screening, 21(4).
+ Open protocol
+ Expand
7

Isolation and Characterization of Bacterial Strains

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ten clinical isolations of S. aureus were collected from patients admitted in the Third Xiangya Hospital of Central South University. S. aureus Newman and ATCC 43300 were provided by Min Li (Shanghai Jiao Tong University, Shanghai, China). Staphylococcus epidermidis RP62A (ATCC 35984) was provided by Di Qu (Fudan University, Shanghai, China). Pseudomonas aeruginosa PAO1 (ATCC 15692) was obtained from Mingqiang Qiao (Nankai University, Tianjin, China). Enterococcus faecalis ATCC 29212, A. baumannii ATCC 1195, Klebsiella pneumoniae ATCC 700603, Escherichia coli ATCC 25922, and S. aureus ATCC 29213 were purchased from the American Type Culture Collection. S. aureus and S. epidermidis strains were grown in tryptic soy broth (TSB) (Solarbio, Beijing, China) medium. E. faecalis strain was cultured in brain–heart infusion broth (Solarbio) medium. Other strains were grown in Luria–Bertani broth (Solarbio) medium.
Zhou L., She P., Tan F., Li S., Zeng X., Chen L., Luo Z, & Wu Y. (2020). Repurposing Antispasmodic Agent Otilonium Bromide for Treatment of Staphylococcus aureus Infections. Frontiers in Microbiology, 11, 1720.
+ Open protocol
+ Expand
8

Microbial Culture Propagation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cultures of Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 43300 (MRSA), Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 700603 and Candida albicans ATCC 64124 were purchased from the American Type Culture Collection (ATCC). The propagation conditions for the isolates were followed as recommended by the ATCC, and isolates were preserved at 4 °C.
Gbala I.D., Macharia R.W., Bargul J.L, & Magoma G. (2022). Membrane Permeabilization and Antimicrobial Activity of Recombinant Defensin-d2 and Actifensin against Multidrug-Resistant Pseudomonas aeruginosa and Candida albicans. Molecules, 27(14), 4325.
+ Open protocol
+ Expand
9

Metallo-beta-Lactamase Producing Isolates

Check if the same lab product or an alternative is used in the 5 most similar protocols
A total of 37 MBL-producing Enterobacterales isolates were selected from either the CDC & FDA Antibiotic Resistance Isolate Bank (n=36) or from CDC’s internal collection of clinical isolates (n=1) (Table 1). MBL genes (blaIMP, blaNDM, or blaVIM) were confirmed by WGS or real-time PCR. All isolates had interpretations of intermediate or resistant for aztreonam (≥8 mg/L) and ceftazidime/avibactam (≥16/4 mg/L) according to CLSI’s M100 30th edition interpretive criteria.13 The following QC strains were used for susceptibility testing: Escherichia coli ATCC 35218, E. coli ATCC 25922, Klebsiella pneumoniae ATCC 700603, Pseudomonas aeruginosa ATCC 27853, and Staphylococcus aureus ATCC 29213 (American Type Culture Collection, Manassas, VA).
Khan B.V., Harrison D.G., Olbrych M.T., Alexander R.W, & Medford R.M. (1996). Nitric oxide regulates vascular cell adhesion molecule 1 gene expression and redox-sensitive transcriptional events in human vascular endothelial cells.. Proceedings of the National Academy of Sciences of the United States of America, 93(17), 9114-9119.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!