Anti gapdh antibody

Fetal bovine serum, Trizol, Lipofectamine 2000and HRP-conjugated mouse and rabbit secondary antibodies were obtained from Invitrogen (Shanghai, China). Endothelial Cell Medium (ECM) was obtained from ScienCell (San Diego, USA). The anti-AXIIR and anti-CD31antibody were obtained from Santa Cruz Biotechnology Co., Ltd. (Dallas, USA). Antibodies to MMP2, MMP9 were obtained from Shanghai RuianBioTechnologies (Shanghai, China). The anti-AKT, anti-p-AKT, anti-ERK, anti-p-ERK, anti-TIMP2 antibodies were purchased from Bioworld Technology, Inc. (Nanjing, China). The anti-GAPDH antibody was obtained from Tianjin sungene Biotech (Tianjin, China). Matrigel Matrix was obtained from BD Biosciences (San Jose, USA). Cell-light EdU DNA cell proliferation kit was obtained from RiboBio (Guangzhou, China). PrimeScript RT reagents Kit, PrimeSTAR® HS DNA Polymerase with GC Buffer and SYBR Premix Ex Taq were obtained from TAKARA BIOTECHNOLOGY (Dalian, China). Annexin V-FITC Apoptosis Detection Kit was obtained from eBioscience (San Diego, CA, USA). The transwell chamber and other cell culture plates were obtained from Corning Inc (NY, USA). All other regents were of analytical grade.