Dihydro s1p

Manufactured by Enzo Life Sciences

Sourced in United Kingdom

Dihydro-S1P is a stable analog of the lipid signaling molecule Sphingosine-1-Phosphate (S1P). It is used in research applications to study the biological functions of S1P.

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Lab products found in correlation

Sphingosine 1 phosphate (s1p), by Enzo Life Sciences (2 mentions) Lysophosphatidic acid, by Merck Group (1 mentions) Ski 2, by Merck Group (1 mentions) Anti β actin antibody, by Merck Group (1 mentions) Vpc23019, by Cayman Chemical (1 mentions) Complete mini protease inhibitor cocktail, by Merck Group (1 mentions) Phosphatidylserine, by Avanti Polar Lipids (1 mentions) Arachidonic acid, by Cayman Chemical (1 mentions) Keap1, by OriGene (1 mentions) β actin, by Merck Group (1 mentions) Ubiquitin, by Merck Group (1 mentions) Sphingosine 1 phosphate (s1p), by Avanti Polar Lipids (1 mentions) β actin, by Abcam (1 mentions) Fty720, by Echelon Biosciences (1 mentions) Fumonisin b1, by Enzo Life Sciences (1 mentions)

2 protocols using dihydro s1p

Regulating Lipid Signaling Pathways

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Phorbol 12, 13-dibutyrate (PDBu), forskolin, calyculin A, and LY294002 were purchased from Calbiochem. EGF was purchased from Upstate Biotechnology. PZ09 was obtained from Reagency. SKI-II, Lysophosphatidic acid (LPA), and cOmplete Mini Protease Inhibitor Cocktail were purchased from Sigma-Aldrich. Caged S1P was purchased from Santa Cruz. VPC23019 was purchased from Cayman Chemical. S1P, dihydro-S1P, Sphingosine, and C16-ceramide were purchased from Enzo Life Sciences. Nitrobenzoxadiazole (NBD)-labeled S1P and phosphatidylserine (PS) were purchased from Avanti Polar Lipids. The anti-PKCζ antibody was purchased from Santa Cruz (C-20, SC-216). The anti-PKCι antibody was purchased from BD (Clone 23, 610176). The anti-β-actin antibody was purchased from Sigma-Aldrich (clone AC-74, A2228). The anti-GFP (Clone mFX75, 012–22541) and anti-GST (Clone 5A7, 013–21851) antibodies were purchased from Wako Pure Chemical Industries. All other materials were reagent-grade.

Kajimoto T., Caliman A.D., Tobias I.S., Okada T., Pilo C.A., Van A.A., McCammon J.A., Nakamura S.I, & Newton A.C. (2019). Activation of Atypical Protein Kinase C by Sphingosine 1-Phosphate Revealed by Atypical Protein Kinase C-Specific Activity Reporter. Science signaling, 12(562), eaat6662.

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Sphingolipids and Antioxidant Pathways

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Sphingosine, SKI-II (2-(p-Hydroxyanilino)-4-(p-chlorophenyl) thiazole), DMS (N,N-Dimethylsphingosine), DHS (dihydro-sphingosine ), FTY720 and SK1-I ((2R,3S,4E)-N-methyl-5-(4′-pentylphenyl)-2-aminopent-4-ene-1,3-diol) were obtained from Echelon Biosciences (Salt Lake City, UT). Hydrogen peroxide,3-(4,5-dimethylthiazol-2 yr)-2-5-diphenyl tetrazoliumbromide (MTT)sulforaphane,CDDO-Imidazolide and propidium were from Sigma (Poole, UK). Dihydrospingosine was from Tocris Bioscience (Bristol, UK). S1P, dihydroS1P and fumonisin B1 were from Enzo Life Sciences International (Exeter, UK). C17s sphingosine, dihydrosphyngosine, S1P and dihydroS1P were purchased from Avanti Polar Lipids (Alabaster, AL). FITC-conjugated anti-macrophage (MOMA2) antibody and anti-neutrophils (7/4) antibody were purchased from Acris Antibodies (GmbH, Herford, Germany). Arachidonic acid and Biotin-Arachidonic acid were purchased from Cayman (Ann Arbor, MI). Neutravidin Agarose Resin was purchased from Thermo Scientist (East Riding of Yorkshire, UK). Antibodies against the following were used for immunoblotting: β-actin, p62, NQO1, ubiquitin (Sigma), Nrf2 (Santa Cruz Biotechnology, Santa Cruz, CA), Nrf2, GCLM, HO-1, TBP, β-actin, SK2 (Abcam, Cambridge, UK), SK1 (Abgent, San Diego, CA) and Keap1 (Origene, Rockville, MD).

Mercado N., Kizawa Y., Ueda K., Xiong Y., Kimura G., Moses A., Curtis J.M., Ito K, & Barnes P.J. (2014). Activation of Transcription Factor Nrf2 Signalling by the Sphingosine Kinase Inhibitor SKI-II Is Mediated by the Formation of Keap1 Dimers. PLoS ONE, 9(2), e88168.

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