Perfection 3200

We measured seven aboveground and fine-root morphological traits related to drought responses and resource use strategies. For aboveground traits, before harvesting, we measured vegetative plant height (Height, cm) and collected one young and fully expanded leaf from three individuals in each pot. For fine-root traits, we collected a sample (10-50mg) of finest roots (<2 mm) from each pot. Leaves and roots were scanned (Epson Perfection 3200 and Epson V700 photo scanner, respectively) and then dried for 72h at 60°C to measure dry leaf and root biomass. Leaf scans were processed with ImageJ software to determine leaf area (LA, mm²). Root scans were processed with WinRHIZO Pro 2015 (Regent Instruments Inc., Canada) to calculate average root diameter (AvgD, mm) and root length (cm). With these measurements, we estimated specific leaf area (SLA, the ratio of fresh leaf area to leaf dry mass, mm² mg^-1) and leaf dry matter content (LDMC, the ratio of leaf dry mass to leaf fresh mass, mg g^-1) for each leaf and averaged the values for each species at the pot level. We also calculated specific root length (SRL, the ratio of root length to root dry mass, cm g^-1) and root dry matter content (RDMC, the ratio of root dry mass to root fresh mass, mg g^-1) for each species at the pot level, due to the complexity associated with disentangling individual roots within a pot.

An inclusion body fraction of semi-purified Nipah N antigen was prepared by negative contrast electron microscopy. Specifically, 20 μl of sample was absorbed to formvar-coated carbon-stabilized copper grids and stained with 2% phosphotungstic acid, pH 7.2 (wt/vol). The specimen grids were examined in a Philips CM 120 transmission electron microscope, operating at an accelerating voltage of 80 kV. Micrographs were taken between 28,000X -45,000X using Kodak Electron Microscope Film 4489. The negatives were scanned using an Epson Perfection 3200 photo scanner and enlarged 2.5X.