Hp 5973 msd mass selective detector

LC-MS analysis of FCME was accomplished by Agilent 1100 series LC system (Agilent Technologies, Santa Clara, CA) equipped with an Agilent 1946B mass selective detector (MSD) (Agilent Technologies, Santa Clara, CA). A Pursuit XRs C18 (2.0 mm × 150 mm, 3 μm) column was utilized at a flow rate of 0.2 mL/min at 40°C. The mobile phase consists of A (0.1% formic acid in water) and B (0.1% formic acid in acetonitrile). The run time for each sample was 60 min, and the mobile phase started with 95% “A” and 5% “B” in the first 2 min; then “A” was decreased gradually to 0% until the 50th min and held for an additional 5 min. Thereafter, the proportion of “A” was increased to 95% within 5 min. The compounds were analyzed in selected ion monitoring (SIM) mode.
GC/MS analysis of FCME was performed by Kyungpook National University Center for Scientific Instruments and carried out using a HP 6890 Plus GC gas chromatograph with a (MSD)—HP 5973 MSD mass selective detector (Hewlett-Packard). Samples were diluted 1 : 1000 (v : v) with HPLC grade dichloromethane. Aliquots of the sample (1 μL) were injected into an HP-5 column. The GC oven temperature was set at 50°C for 4 min, increased to 280°C at a rate of 4°C/min, and held at the final temperature for 2 min. Velocity of the He carrier gas (99.99%) was 0.7 mL/min. Quantitative analysis was performed using the area normalization method.

In order to understand the differences between classical industrial and soft organic fabrics, cotton and flax extracts were analyzed by high performance liquid chromatography (HPLC) coupled to ultra-violet detection (Thermo Fisher Scientific U3000RS HPLC pump equipped with UV-DAD detector set at 220 nm). For that, 1 mL of each sample was submitted to extraction using 49 mL methanol/water (50/50 v/v) with ortho-phosphoric acid 0.085% (Sigma-Aldrich, Darmstadt, Germany). After 2 h incubation under constant agitation (300 rpm), the solution was filtrated on 0.45 µm regenerated cellulose syringe filters (Thermo Fisher Scientific, Illkirch-Graffenstaden, France). Separation was realized by gradient elution using methanol/water with 0.085% ortho-phosphoric acid (from 5/95 to 75/25 v/v) and a RP NUCLEODUR C18 Isis 5 µm 250 × 4 mm Machery Nagel column. A molecule of interest was further identified by gas chromatograph coupled to mass spectrometry using a HP Hewlett Packard Agilent 6890 Plus GC System Gas Chromatograph with HP 5973 MSD Mass Selective Detector.