Enhanced chemiluminescence ecl immunoblot detection reagents

Manufactured by Thermo Fisher Scientific

Enhanced chemiluminescence (ECL) immunoblot detection reagents are laboratory products used to detect and visualize specific proteins in Western blot analysis. These reagents utilize a chemiluminescent reaction to produce a signal that can be captured and quantified, allowing for the identification and measurement of target proteins.

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Lab products found in correlation

N biotinoyl n iodoacetyl ethylenediamine biam, by Thermo Fisher Scientific (2 mentions) Aprotinin, by Merck Group (2 mentions) Leupeptin, by Merck Group (2 mentions) Bestatin, by Merck Group (2 mentions) Sec498cys, by AbFrontier (1 mentions) Horseradish peroxidase hrp conjugated streptavidin, by GE Healthcare (1 mentions) Ethylene diamine tetraacetic acid (edta), by Merck Group (1 mentions) Insulin, by Merck Group (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) Phosphatase inhibitor, by Merck Group (1 mentions) Nadph, by Merck Group (1 mentions) 5 5 dithiobis 2 nitrobenzoic acid, by Merck Group (1 mentions) Napqi, by Merck Group (1 mentions)

Close spelling variants of this product

Enhanced chemiluminescence (ECL) detection reagent Enhanced chemiluminescence (ECL) reagent Enhanced chemiluminescence detection reagents ECL chemiluminescence detection reagent Enhanced chemiluminescence reagent Enhanced ECL reagent Enhanced chemiluminescence (ECL) ECL chemiluminescence reagent Enhanced chemiluminescence detection Chemiluminescence detection reagents

2 protocols using enhanced chemiluminescence ecl immunoblot detection reagents

Enzymatic Assays for Oxidative Stress

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Purified rat liver TrxR1, recombinant
human Trx1, and glutathione reductase (GR), recombinant E.
coli Trx1, bovine glutathione peroxidase (GPx), insulin,
NADPH, 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), APAP, NAPQI,
menadione (2-methyl-1,4-napthoquinone), reduced glutathione (GSH),
oxidized glutathione (GSSG), phosphatase inhibitors (catalog no. P2850,
which contains microcystinLR, cantharidin, and (−)-p-bromotetramisole), and protease inhibitor cocktail (catalog
no. P2714, which contains 4-(2-aminoethyl)benzenesulfonyl fluoride,
E-64, bestatin, leupeptin, aprotinin, and EDTA) were purchased from
Sigma-Aldrich (St. Louis, MO). Sodium aurothiomalate hydrate was from
Aldrich (Milwaukee, WI). Human recombinant TrxR mutant enzyme (Sec498Cys)
was from AbFrontier (Seoul, Korea). Pooled human liver microsomes
(HLM) and recombinant human P450s 1A2, 2E1, and 3A4 were purchased
from BD Genetest (Woburn, MA). N-(Biotinoyl)-N′-(iodoacetyl) ethylenediamine (BIAM) was from Molecular
Probes (Eugene, OR) and horseradish peroxidase (HRP)-conjugated streptavidin
from GE Healthcare (Piscataway, NJ). Enhanced chemiluminescence (ECL)
immunoblot detection reagents were from Thermo Scientific (Rockford,
IL).

Jan Y.H., Heck D.E., Dragomir A.C., Gardner C.R., Laskin D.L, & Laskin J.D. (2014). Acetaminophen Reactive Intermediates Target Hepatic Thioredoxin Reductase. Chemical Research in Toxicology, 27(5), 882-894.

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Enzyme Kinetics of Redox Regulators

Check if the same lab product or an alternative is used in the 5 most similar protocols

Purified rat liver TrxR1, recombinant human Trx1 and glutathione
reductase (GR), recombinant E. coli Trx1, bovine glutathione
peroxidase (GPx), insulin, NADPH, 5,5′-dithiobis(2-nitrobenzoic acid)
(DTNB), APAP, NAPQI, menadione (2-methyl-1,4-napthoquinone), reduced glutathione
(GSH), oxidized glutathione (GSSG), phosphatase inhibitors (catalog no. P2850,
which contains microcystinLR, cantharidin, and
(−)-p-bromotetramisole) and protease inhibitor cocktail
(catalog no. P2714, which contains 4-(2-aminoethyl)benzenesulfonyl fluoride,
E-64, bestatin, leupeptin, aprotinin, and EDTA) were purchased from
Sigma-Aldrich (St. Louis, MO). Sodium aurothiomalate hydrate was from Aldrich
(Milwaukee, WI). Human recombinant TrxR mutant enzyme (Sec498Cys) was from
AbFrontier (Seoul, Korea). Pooled human liver microsomes (HLM) and recombinant
human P450s 1A2, 2E1, and 3A4 were purchased from BD Genetest (Woburn, MA).
N-(Biotinoyl)-N′-(iodoacetyl)
ethylenediamine (BIAM) was from Molecular Probes (Eugene, OR) and horseradish
peroxidase (HRP)-conjugated streptavidin from GE Healthcare (Piscataway, NJ).
Enhanced chemiluminescence (ECL) immunoblot detection reagents were from Thermo
Scientific (Rockford, IL).

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