Ethyl bromide

Manufactured by Merck Group

Sourced in Germany, Poland

Ethyl bromide is a chemical compound with the formula C2H5Br. It is a colorless, volatile liquid used as a laboratory reagent and chemical intermediate.

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Lab products found in correlation

Close spelling variants of this product

Lidocaine N-ethyl bromide (QX-314 (4 citations) Lidocaine N-ethyl bromide (3 citations) 1-ethyl-1-methyl pyrrolidinium bromide (2 citations) 2-ethyl hexyl bromide (2 citations)

7 protocols using ethyl bromide

Telomere Length Measurement Using Southern Blot

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Telomere length in each of the tissues was assessed using TeloTAGGG^™ Telomere Length Assay (Sigma-Aldrich, St Louis, MO) as per manufacturer’s recommendation and described elsewhere (Tarry-Adkins and Ozanne 2018 (link)). Briefly, about 1.0 μg DNA was digested with HinfI and RsaI restriction enzymes for 2 h at 37 °C and the restriction enzyme reaction quenched by adding 5× SDS loading buffer (Roche Diagnostics, Mannheim, Germany). The samples were than loaded onto agarose gels containing ethyl bromide (Sigma-Aldrich) and separated by pulsed field gel electrophoresis. The undigested and digested DNA were checked for non-specific degradation and digestion by the restriction enzymes by visualizing under UV light (BioRad). The separated DNA fragments were transferred to nylon membrane (Roche Diagnostics) by Southern blotting, and dioxygenin labelled telomeric repeat length was determined with anti- dioxygenin antibodies using chemiluminescent detection. The densitometry of the telomere signals was analyzed using ImageJ (National Institutes of Health, Bethesda, MD) as described previously (Tarry-Adkins and Ozanne 2018 (link)).

Puttabyatappa M., Ciarelli J.N., Chatoff A.G, & Padmanabhan V. (2021). Developmental Programming: Metabolic Tissue-Specific Changes in Endoplasmic Reticulum Stress, Mitochondrial Oxidative and Telomere Length Status induced by Prenatal Testosterone Excess in the Female Sheep. Molecular and cellular endocrinology, 526, 111207.

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Synthesis and Characterization of Carbazolyl Derivatives

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Commercially available hexaazatriphenylenehexacabonitrile (HAT-CN), tris(4-carbazoyl-9-ylphenyl)amine (TCTA), 2,3,5,6-tetrakis(3,6-diphenyl-9H-carbazol-9-yl)-1,4- benzenedicarbonitrile (4CzTPN-Ph), bis(1-phenyl-isoquinoline-C2,N)(acetylacetonato)iridium(III) (Ir(piq)₂ (acac)), 2,4,6-tris[3-(diphenylphosphinyl)phenyl]-1,3,5-triazine (PO-T2T), 2,2′,2′’-(1,3,5-benzinetriyl)-tris(1-phenyl-1-H-benzimidazole) (TPBi), fluorolithium (LiF) were purchased from Sigma-Aldrich and used as received.
The starting materials i.e. 9H-carbazole, sodium tert-butoxide, tetrakis-triphenylphosphine palladium (Pd(PPh₃)₄), ethyl bromide, glacial acetic and sulfuric acids, copper (Cu), potassium iodide (KI), potassium iodate (KIO₃), sodium hydroxide (NaOH), potassium carbonate (K₂CO₃), anhydrous sodium sulfate (Na₂SO₄), potassium hydroxide (KOH), copper cyanide (CuCN), 18-crown-6, and 1,2-dichlorobenzene were purchased from Sigma-Aldrich and used as received. 9-Ethyl-9H-carbazole was synthesized according to the previously reported procedure [27] (link). 9-Ethyl-3,6-diiodo-9H-carbazole was obtained similarly to the known procedure [28] (link). Synthetic procedures and the structural characterization of the cyanocarbazolyl derivatives are described in Supporting Information.
In addition, procedures of the provided experiments are presented in Supporting Information (see section Instrumentation).

Sych G., Guzauskas M., Volyniuk D., Simokaitiene J., Starykov H, & Grazulevicius J.V. (2020). Exciplex energy transfer through spacer: White electroluminescence with enhanced stability based on cyan intermolecular and orange intramolecular thermally activated delayed fluorescence. Journal of Advanced Research, 24, 379-389.

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Synthesis and Characterization of PLA-PGGA Blends

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Semicrystalline polylactic acid (PLA), Mw ≈ 140,000 g/mol; Poly(γ-glutamic acid) (PGGA) was supplied by Meiji Co., (Tokyo, Japan). The following reactants were purchased at Sigma-Aldrich Company Ltd.: 1-dodecanol (CH₃(CH₂)₁₁OH), >98%; α-tocopherol (C₂₉H₅₀O₂); ethyl bromide (C₂H₅Br), 98%; titanium tetrabutoxide (Ti(OBu)₄), 97%; iron (II) chloride tetrahydrate (FeCl₂·4H₂O), 99%; alumina (aluminum oxide) (Al₂O₃), 98%; hydrochloric acid (HCl), 37%; deuterated chloroform (CDCl₃), 99% D atoms; trichloroacetic acid (TCA) (Cl₃CCOOH), 99%; trifluoroacetic acid (TFA) (CF₃COOH), 99%; trimethylchlorosilane ((CH₃)₃SiCl), >98%; sodium carbonate (Na₂CO₃), >99%.
Sodium bicarbonate (NaHCO₃), 99%; Polyoxyethylene (20) sorbitan monolaurate (Tween-20) (C₅₈H₁₁₄O₂₆); solvent N-methylpyrrolidone (NMP) (C₅H₁₁N), 97%; and chloroform (CHCl₃), >99%, were bought at Merck (Darmstadt, Germany). Ammonium thiocyanate (NH₄SCN), 98%; toluene (C₆H₅CH₃), >98%, were purchased at Panreac Química S.L.U (Barcelona, Spain). Ethanol (CH₃CH₂OH), 96%, was purchased at Solvech. Sunflower oil was purchased from a local supermarket.

Villasante J., Codina E., Hidalgo G.I., Martínez de Ilarduya A., Muñoz-Guerra S, & Almajano M.P. (2019). Poly (α-Dodecyl γ-Glutamate) (PAAG-12) and Polylactic Acid Films Charged with α-Tocopherol and Their Antioxidant Capacity in Food Models. Antioxidants, 8(8), 284.

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Synthesis of Heterocyclic Compounds

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Phthalimide, 2-hydroxy-1-naphthaldehyde, salicylaldehyde, ethyl bromide (EtBr), and trifluoroacetic acid (TFA) were purchased from Sigma-Aldrich and used without further purification. Nitric acid, sulphuric acid, potassium carbonate, N,N-dimethylformamide (DMF), and methanol (MeOH) were delivered from local supplier Valerus Ltd.
For spectroscopy measurements, all solvents were of spectroscopy grade purity.

Yordanov D., Deneva V., Georgiev A., Crochet A., Fromm K.M, & Antonov L. (2020). Indirect solvent assisted tautomerism in 4-substituted phthalimide 2-hydroxy-Schiff bases. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 237.

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Synthesis of Substituted Benzene Dicarboxylates

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Materials and reagents were obtained from multiple
different commercial
suppliers and used without further purification, specifically: dimethoxy-2,5-dihydroxybenzenedicarboxylate
(Sigma-Aldrich, 99+%), methyl iodide (Alfa Aesar, 99%, stabilized
with copper), ethyl bromide (Sigma-Aldrich, 98%), propyl bromide (Sigma-Aldrich,
99%), butyl bromide (Acros, 99%), pentyl bromide (Alfa Aesar, 99%),
copper nitrate trihydrate (SLS, 98%), acetonitrile (Fisher, ≥
99.9%) and n,n-dimethylformamide
(Fisher, ≥99%).

Ashworth D.J., Roseveare T.M., Schneemann A., Flint M., Dominguez Bernáldes I., Vervoorts P., Fischer R.A., Brammer L, & Foster J.A. (2019). Increasing Alkyl Chain Length in a Series of Layered Metal–Organic Frameworks Aids Ultrasonic Exfoliation to Form Nanosheets. Inorganic Chemistry, 58(16), 10837-10845.

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Synthesis and Characterization of Polymers in AAO Templates

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2,2′-azobis(2-methylpropionitrile) (AIBN, 0.2 M solution in toluene), 2-(dodecylthiocarbonothioylthio)propionic acid (CTA), 1-vinylimidazole, iodomethane, ethyl bromide, dimethyl sulfoxide-d₆ and LiNTf₂ were purchased from Sigma Aldrich. The nanoporous aluminum oxide membranes of d = 150 nm used in this study were supplied from Synkera Co. On the other hand, AAO membranes with the pore diameter of d = 35 nm were prepared by two-step anodization of aluminum in stirred 0.3 M H₂C₂O₄ at 25 °C.^{27 (link)} The chemical structure of investigated compounds is presented in Fig. 1 together with the scheme of applied AAO templates. Details concerning properties of AAO templates can be found in the ESI.†

Maksym P., Tarnacka M., Dzienia A., Wolnica K., Dulski M., Erfurt K., Chrobok A., Zięba A., Brzózka A., Sulka G., Bielas R., Kaminski K, & Paluch M. (2019). Efficient metal-free strategies for polymerization of a sterically hindered ionic monomer through the application of hard confinement and high pressure. RSC Advances, 9(11), 6396-6408.

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Polymer Synthesis and Characterization

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1,2-Dichlorobenzene (99%), 1,1,4,7,10,10-hexamethyltriethylenetetramine (HMTETA, 97%), 2-bromopropionitrile (97%), copper (I) bromide (CuBr, 99.999%), copper (II) bromide (CuBr₂, 99%), p-xylene (≥99%) and ethyl bromide (98%) were purchased from Sigma Aldrich (Poznan, Poland) and used as received. N,N′-dimethylaminoethyl methacrylate (DMAEMA, 98%) was purchased from Sigma Aldrich and purified by distillation prior to use. An ion exchanger DOWEX MARATHON MSC was purchased from Sigma Aldrich and transformed into H⁺ using 1.6 mol/L HNO₃. Acetone (99.5%) and silver nitrate (AgNO₃, 99.9%) were purchased from POCH (Gliwice, Poland) and used as received. Tetrahydrofuran (THF, pure p.a.) was purchased from POCH and purified by distillation prior to use. A SpectraPor membrane with MWCO 1000 g/mol was purchased from Roth (Karlsruhe, Germany). Phosphate buffer saline (PBS) was purchased from PAA Laboratories GmbH (Oberosterreich, Austria). Mueller Hinton Broth (MHB) was purchased from HiMedia (Mumbai, India).

Teper P., Sotirova A., Mitova V., Oleszko-Torbus N., Utrata-Wesołek A., Koseva N., Kowalczuk A, & Mendrek B. (2020). Antimicrobial Activity of Hybrid Nanomaterials Based on Star and Linear Polymers of N,N′-Dimethylaminoethyl Methacrylate with In Situ Produced Silver Nanoparticles. Materials, 13(13), 3037.

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