Antibody against inos

Oviducts Ranae were collected from the adult female Chinese brown frogs (obtained during the prehibernation from Jilin Baekdu Mountain Chinese Brown Frog Breeding Farm, Jilin Province, China). All the procedures on animals were carried out in accordance with the Policy on the Care and Use of Animals by the Ethical Committee, Beijing Forestry University. Murine macrophage cell line RAW 264.7 (ATCC number TIB-71) and cell culture materials were purchased from the Cell Culture Centre of the Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing, China. Papain, trypsin, pepsin, neutral protease, and alkaline protease were purchased from Sigma. Griess reagent and nuclear (or cytoplasmic) protein extraction kit were obtained from Beyotime Biotechnology, China. Antibody against iNOS was from Santa Cruz Biotechnology Inc. (Dallas, TEX, USA). ELISA kits (for TNF-α, IL-1β, and IL-6) were purchased from Cusabio Biotech, China. Anti-NF-κBp65 and anti-IκB-α were obtained from Biosynthesis Biotechnology, China.

Eupatin was obtained from the National Cancer Institute (NCI No. 122412). We purchased primary antibodies against p-GSK3β (Tye216) and p-p65 (Ser536) from Cell Signaling Technology (Danvers, MA, USA). We obtained antibodies against p-tau (Ser396) from Abcam (Cambridge, MA, USA) and those against p-tau (Ser202/Thr205), p-tau (Ser262), and tau w from Thermo Fisher Scientific (Waltham, MA, USA). Antibodies against GSK3β and α-tubulin were obtained from GeneTex Inc. (Hsinchu, Taiwan), and antibody against iNOS was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Jackson ImmunoResearch Inc. (West Grove, PA, USA) was our source for horseradish peroxidase (HRP)-conjugated antimouse and antirabbit immunoglobulin G secondary antibodies, and OA was purchased from Cayman Chemical Company (Ann Arbor, MI, USA). Unless otherwise stated, we obtained all chemicals not mentioned here from Sigma-Aldrich (St. Louis, MO, USA).