A-375 (#300110) and U-251 MG (#300385) cell lines were purchased from CLS-cell line service (Eppelheim, Germany). IPC-298 (#ACC 251) cells were purchased from DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen; Braunschweig, Germany. Except for U-251 MG, all cell lines were positive for CSPG4. The cell lines were grown at 37°C in a humidified atmosphere containing 5% CO2 in media recommended by the manufacturer. All cell culture media and reagents were purchased from GE Healthcare (Buckinghamshire, GB).
Cell culture media and reagents
Manufactured by GE Healthcare
Sourced in Austria
Cell culture media and reagents are essential components for the growth, maintenance, and manipulation of cells in a laboratory setting. They provide the necessary nutrients, growth factors, and other essential elements required for the proliferation and viability of cells in culture. These products support a wide range of cell types, including mammalian, insect, and microbial cells, and are used in various applications such as cell-based assays, gene expression studies, and tissue engineering.
Automatically generated - may contain errors
Lab products found in correlation
Gel green, by Biotrend (2 mentions)
Anti mouse cd45 30 f11, by BD (1 mentions)
Bio gel polyacrylamide beads p 100 fine 45 90 μm, by Bio-Rad (1 mentions)
Fetal bovine serum (fbs), by Harvard Bioscience (1 mentions)
Lovastatin, by Merck Group (1 mentions)
Human insulin, by Merck Group (1 mentions)
Resveratrol, by Merck Group (1 mentions)
Gelred, by Biotrend (1 mentions)
4 protocols using cell culture media and reagents
1
Characterization of Commonly Used Cancer Cell Lines
2
Myeloid Cell Isolation and Characterization
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Bio-gel polyacrylamide beads (P-100 fine, 45–90 μm) were purchased from Bio-Rad (Hercules, CA, USA); anti-mouse CD45.1 (A20), CD45.2 (104), CD11b (M1/70), CD115 (AFS98), lymphocyte antigen 6 complex, locus C (Ly-6C) (HK1.4), and lymphocyte antigen 6 complex, locus G (Ly-6G) (1A8) were purchased from BioLegend (San Diego, CA, USA); anti-mouse CD45 (30-F11) was obtained from BD Biosciences (San Jose, CA, USA); quantitative PCR (qPCR) primers were purchased from Qiagen (Germantown, MD, USA); and all cell culture media and reagents were obtained from GE Healthcare (Waukesha, WI, USA).
3
Cell Culture Reagent Procurement Protocol
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Unless stated otherwise, chemicals were purchased from Sigma Aldrich Germany (Munich, Germany). Cell culture media and reagents were purchased from PAA Laboratories (Pasching, Austria) and Life Technologies (Darmstadt, Germany). Fetal bovine serum (FBS) was from Biochrom (Berlin, Germany). Microscope slides were from Asisstent (Sondheim, Germany) and methanol was from thgeyer (Renningen, Germany). Gel Green was purchased from Biotrend (Köln, Germany).
4
Insulin, Lovastatin, and Resveratrol Protocol
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Human insulin, lovastatin and resveratrol were purchased from Sigma-Aldrich (St. Louis, MO, USA, or Munich, Germany). RAD-001 was purchased from MedChemTronica (Stockholm, Sweden). Gel Red and Gel Green were purchased from Biotrend (Köln, Germany). Cell culture media and reagents were obtained from PAA Laboratories GmbH (Pasching, Austria) and Invitrogen Life Technologies (Carlsbad, CA, USA, or Darmstadt, Germany). Anti-AKT (pS473) antibody (22650) was purchased from Rockland (Gilbertsville, PA, USA). Anti ß-actin antibody (T6199) was purchased from Sigma-Aldrich (Taufkirchen, Germany).
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