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Recombinant hifnα

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Recombinant hIFNα is a human interferon alpha protein produced in a recombinant expression system. Interferons are a class of signaling proteins involved in the body's immune response.

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Lab products found in correlation

Recombinant hifn γ, by R&D Systems (2 mentions) Lna14, by R&D Systems (2 mentions) Poly 1 c, by R&D Systems (2 mentions) Thp1 dual cells, by InvivoGen (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Htnf α, by R&D Systems (1 mentions)

2 protocols using recombinant hifnα

1

Cytokine Induction by Innate Immune Stimuli

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Supernatants from THP-1-Dual cells (InvivoGen) were transfected in triplicates with LNA9, LNA14 or poly (I:C) (R&D Systems). Treatment with recombinant hIFNγ (R&D Systems), recombinant hIFNα (R&D Systems) or LPS from O111:B4 E. Coli (Sigma Alderich) were used as positive control to indicteinduction of IL-6 or TNF-α secretion. Supernatants were collected 24 hours post transfection/treatment and assayed for TNF-α and IL-6 concentration by ELISA (ThermoFisher) accordign to the ELISA kit protocol.
Hagey R.J., Elazar M., Pham E.A., Tian S., Ben-Avi L., Bernardin-Souibgui C., Yee M., Moreira F.R., Rabinovich M., Meganck R.M., Fram B., Beck A., Gibson S.A., Lam G., Devera J., Kladwang W., Nguyen K., Xiong A., Schaffert S., Avisar T., Liu P., Rustagi A., Fichtenbaum C.J., Pang P.S., Khatri P., Tseng C.T., Taubenberger J.K., Blish C.A., Hurst B.L., Sheahan T.P., Das R, & Glenn J.S. (2022). Programmable antivirals targeting critical conserved viral RNA secondary structures from influenza A virus and SARS-CoV-2. Nature medicine, 28(9), 1944-1955.
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2

Dual Reporter Assay for IFN and NF-kB

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THP1-Dual or A549-Dual cells (InvivoGen) were transfected in triplicates with LNA9, LNA14 or derivatives theroff, or poly(I:C) (R&D Systems) using Lipofectamine 2000 (ThermoFisher) according to the manufacturer protocol. Treatment with rcombinant hTNF-α (R&D Systems), recombinant hIFN-γ (R&D Systems), recombinant hIFN-α (R&D Systems) or LPS from O111:B4 E. Coli (Sigma Alderich) were used as positive control for activation of the two pathways. Supernatant from the cultures were collected 24–32 hours post transfection/treatment to measure luciferase signal or SEAP activity as indicators of interferon pathways stimulation or NF-kB pathway stimulation respectively. Results were normalized to reflect fold induction relative to NT cells.
Hagey R.J., Elazar M., Pham E.A., Tian S., Ben-Avi L., Bernardin-Souibgui C., Yee M., Moreira F.R., Rabinovich M., Meganck R.M., Fram B., Beck A., Gibson S.A., Lam G., Devera J., Kladwang W., Nguyen K., Xiong A., Schaffert S., Avisar T., Liu P., Rustagi A., Fichtenbaum C.J., Pang P.S., Khatri P., Tseng C.T., Taubenberger J.K., Blish C.A., Hurst B.L., Sheahan T.P., Das R, & Glenn J.S. (2022). Programmable antivirals targeting critical conserved viral RNA secondary structures from influenza A virus and SARS-CoV-2. Nature medicine, 28(9), 1944-1955.
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