Log In Sign up
The largest database of trusted experimental protocols

Kapa sybr fast qpcr 2x master mix

Manufactured by New England Biolabs
Visit Supplier

KAPA SYBR FAST qPCR 2x Master Mix is a reagent for quantitative polymerase chain reaction (qPCR) assays. It contains all the necessary components, including a DNA polymerase, buffer, and SYBR Green I dye, for the amplification and detection of DNA targets.

Automatically generated - may contain errors

Lab products found in correlation

Direct zol rna miniprep kit, by Zymo Research (2 mentions) Lunascript rt supermix kit, by New England Biolabs (2 mentions)

Close spelling variants of this product

QPCR Master Mix

2 protocols using kapa sybr fast qpcr 2x master mix

1

Gene Expression Analysis of Treated Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were plated at sub-confluence and the next day media was changed to contain the appropriate treatment (ex. 500 nM dTAG47 or 0.01% DMSO vehicle control). Cells were collected in Trizol and RNA was purified with Direct-Zol RNA Miniprep kit (Zymo) with on-column DNaseI treatment. RNA was reverse transcribed with LunaScript RT SuperMix Kit (New England Biolabs) and analyzed by qPCR using gene-specific primers (Table S6) and KAPA SYBR FAST qPCR 2x Master Mix.
Guarnaccia A.D., Rose K.L., Wang J., Zhao B., Popay T.M., Wang C.E., Guerrazzi K., Hill S., Woodley C.M., Hansen T.J., Lorey S.L., Shaw J.G., Payne W.G., Weissmiller A.M., Olejniczak E.T., Fesik S.W., Liu Q, & Tansey W.P. (2021). Impact of WIN site inhibitor on the WDR5 interactome. Cell reports, 34(3), 108636.
+ Open protocol
+ Expand
2

Gene Expression Analysis of Treated Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were plated at sub-confluence and the next day media was changed to contain the appropriate treatment (ex. 500 nM dTAG47 or 0.01% DMSO vehicle control). Cells were collected in Trizol and RNA was purified with Direct-Zol RNA Miniprep kit (Zymo) with on-column DNaseI treatment. RNA was reverse transcribed with LunaScript RT SuperMix Kit (New England Biolabs) and analyzed by qPCR using gene-specific primers (Table S6) and KAPA SYBR FAST qPCR 2x Master Mix.
Guarnaccia A.D., Rose K.L., Wang J., Zhao B., Popay T.M., Wang C.E., Guerrazzi K., Hill S., Woodley C.M., Hansen T.J., Lorey S.L., Shaw J.G., Payne W.G., Weissmiller A.M., Olejniczak E.T., Fesik S.W., Liu Q, & Tansey W.P. (2021). Impact of WIN site inhibitor on the WDR5 interactome. Cell reports, 34(3), 108636.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!