Il 6 elisa kit

The human colorectal cancer LoVo cells were purchased from ATCC (Manassas, VA, USA). LoVo cells were cultured in F12K medium supplemented with 10% fetal bovine serum, 100 g/mL streptomycin, and 100 U/mL penicillin, at 37°C, 5% CO₂, and high humidity. The sources of antibodies (Abs) were as follows: IL-6 was purchased from R&D (Minneapolis, MN, USA), p-STAT3 was purchased from Abcam (Cambridge, MA, USA), cleaved caspase-3 was purchased from Cell Signaling (Beverly, MA, USA), and STAT3, cyclin D1, GAPDH, and the HRP-labeled secondary antibodies were purchased from EnoGene (Nanjing, China). Carboplatin was purchased from MelonePharma (Dalian, Liaoning, China). Annexin-V-FITC apoptosis detection kit, DAB Substrate Kit, and Cell Counting Kit-8 (CCK-8) were purchased from Beyotime (Beyotime, Shanghai, China). IL-6 ELISA kit was from NeoBioscience (Shenzhen, Guangdong, China).

Aβ42 and XD4 peptide were synthesized from Chinese Peptide Company (Hangzhou, China). Both Aβ40 and Aβ42 kits for Aβ measurement were purchased from Immuno-Biological Laboratories Co., Ltd. (Gunma, Japan). TNF-α, IL-1β and IL-6 ELISA kits were obtained from Neobioscience Technology Co., Ltd. (Beijing, China). The following antibodies were used: 6E10 (monoclonal raised against Aβ1-16, Signet, SIG39300), W20 (oligomer-specific antibody, developed and prepared in our laboratory), anti-Iba-1 antibody (GenTex, GTX100042), anti-GFAP antibody (Abcam, ab53554), anti-PSD-95 antibody (Abcam, ab18258), anti-synaptophysin antibody (Abcam, ab32127), anti-COX2 antibody (Abcam, ab179800), anti-iNOS antibody (Abcam, ab178945), anti-GAPDH antibody (CST, 2118S), goat anti-rabbit secondary antibody conjugated to Alexa Fluor 488 (Santa Cruz, I1112) or Alexa Fluor 594 (Abcam, ab150084), HRP-conjugated goat anti-mouse or rabbit IgG antibody (Zhongshan Golden Bridge Biotechnology, Beijing, China).

CdCl₂ was obtained from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China), with a purity of >98.0%. Alanine/aspartate aminotransferase (ALT/AST) kits were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). T-PER tissue extraction reagent, NE-PER nuclear and cytoplasmic extraction reagents, SuperSignal West Pico PLUS Substrate kit, and Pierce BCA Protein Assay Kit were obtained from ThermoFisher Scientific (Waltham, MA, USA). Murine IL-1β and IL-6 ELISA kits were purchased from Neobioscience (Shenzhen, China). The antibodies that were used for immunoblotting anti-Keap1, -p38, -phospho-p38, -ERK, -phospho-ERK, -JNK, -phospho-JNK, -NLRP3, -NF-κB p65, and -phospho- NF-κB p65 were purchased from Cell Signaling Technology (Danvers, MA, USA) (1:1000 dilutions). The antibodies against Nrf2 and HO-1 were all purchased from Santa Cruz (Santa Cruz, CA, USA) (all 1:200 dilutions). Antibodies against GAPDH were purchased from Abways Technology (Shanghai, China) (1:2000 dilutions). Peroxidase-conjugated goat anti-rabbit immunoglobulin IgG (H + L), anti-mouse IgG (H + L), and anti-goat IgG (H + L) were obtained from Proteintech Group (Wuhan, China). Other reagents, unless indicated, were obtained from Sigma Chemical Co. (St. Louis, MO, USA).