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Rhod 2 tripotassium salt

Manufactured by AAT Bioquest
Sourced in United States

Rhod-2 tripotassium salt is a fluorescent calcium indicator that is used for the detection and measurement of intracellular calcium levels. It is a water-soluble dye that can be loaded into cells and used for real-time monitoring of calcium dynamics.

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2 protocols using rhod 2 tripotassium salt

1

Optogenetic Manipulation of Neuronal Signaling

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Anti-vGAT-Oyster 550 antibody was purchased from Synaptic System (Goettingen, Germany). Anti-HA antibody was from Roche (Milan, Italy). BAPTA (1,2-bis(o-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid), L-NAME (L-NG-Nitroarginine methyl ester), Nifedipine (1,4-Dihydro-2,6-dimethyl-4-(2-nitrophenyl)-3,5-pyridinedicarboxylic acid dimethylester), and Bicuculline were purchased from Sigma (Milan, Italy). KN-93 and KN-92 were acquired from Millipore Merck (Darmstadt, Germany). APV (D-(−)-2-Amino-5-phosphonopentanoic acid), CNQX (6-Cyano-7-nitroquinoxaline-2,3-dione), ω-conotoxin MVIIC, ω-conotoxin GVIA, DPNI-caged-GABA (1-(4-Aminobutanoyl)-4-[1,3-bis(dihydroxyphosphoryloxy)propan-2-yloxy]-7-nitroindoline) and MNI-caged-L-glutamate ((S)-α-amino-2,3-dihydro-4-methoxy-7-nitro-δ-oxo-1H-indole-1-pentanoic acid) were purchased from Tocris (Bristol, UK). Rhod-2 tripotassium salt was purchased from AAT Bioquest (Sunnyvale, CA, USA).
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2

Immunochemicals for Neurophysiology Experiments

Check if the same lab product or an alternative is used in the 5 most similar protocols
Anti-vGAT-Oyster 550 antibody was purchased from Synaptic System (Goettingen, Germany).
Anti-HA antibody was from Roche (Milan, Italy). BAPTA (1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid), L-NAME (L-NG-Nitroarginine methyl ester), Nifedipine (1,4-Dihydro-2,6-dimethyl-4-(2-nitrophenyl)-3,5-pyridinedicarboxylic acid dimethylester), and Bicuculline were purchased from Sigma (Milan, Italy). KN-93 and KN-92 were acquired from Millipore Merck (Darmstadt, Germany). APV (D-(-)-2-Amino-5-phosphonopentanoic acid), CNQX (6-Cyano-7nitroquinoxaline-2,3-dione), ω-conotoxin MVIIC, ω-conotoxin GVIA, DPNI-caged-GABA (1-(4-Aminobutanoyl)-4-[1,3-bis(dihydroxyphosphoryloxy)propan-2-yloxy]-7-nitroindoline) and MNIcaged-L-glutamate ((S)-α-amino-2,3-dihydro-4-methoxy-7-nitro-δ-oxo-1H-indole-1-pentanoic acid) were purchased from Tocris (Bristol, UK). Rhod-2 tripotassium salt was purchased from AAT Bioquest (Sunnyvale, CA, USA).
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