Ab15580

Manufactured by Agilent Technologies

The Ab15580 is a laboratory equipment product from Agilent Technologies. It is designed for general laboratory applications, but a detailed description cannot be provided while maintaining an unbiased and factual approach without extrapolation.

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Lab products found in correlation

M0879, by Agilent Technologies (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Prolactin, by Merck Group (1 mentions) Goat anti guinea pig igg alexa fluor 555, by Thermo Fisher Scientific (1 mentions) Fluor save mounting medium, by Avantor (1 mentions) Axiovision fluorescence microscope, by Zeiss (1 mentions) Alexa fluor 488 goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)

2 protocols using ab15580

Immunohistochemical Analysis of Proliferation Markers

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The expression of proliferation- associated antigens Ki67 and proliferating cell nuclear antigen (PCNA) in tissues was measured by immunohistochemistry assay. After conventional paraffin embedding, sectioning, dewaxing and hydration, the tumor tissues were treated with antigen repair buffer (pH 6.0). Following treatment with 0.3% H₂O₂ for 20 min, the tumor tissue sections were blocked with 5% BSA (Sigma-Aldrich; Merck KGaA) and incubated with primary antibodies (anti-Ki-67, Abcam, ab15580, 1:200 dilution; anti-PCNA, Dako, M0879, 1:50 dilution) overnight at 4°C. On the following day, the tissue sections were washed with PBS three times and incubated with secondary antibodies at 37°C for 1 h, followed by treatment with DAB chromogen.

Zhang R., Zhu H., Yuan Y., Wang Y, & Tian Z. (2020). SPAG6 promotes cell proliferation and inhibits apoptosis through the PTEN/PI3K/AKT pathway in Burkitt lymphoma. Oncology Reports, 44(5), 2021-2030.

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Measuring Proliferation of Pancreatic β-Cells

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Proliferative β-cells were detected by immunostaining of transfected islet cells with rabbit anti-Ki67 (Abcam ab15580, 1:1500), guinea pig anti-insulin (Dako A0564, 1:100), goat anti-rabbit IgG Alexa Fluor 488 (Thermo Fisher A-11008, 1:500), and goat anti-guinea pig IgG Alexa Fluor 555 (Thermo Fisher A-21435, 1:500) antibodies. Coverslips were mounted on microscope glass slides with Fluor-Save mounting medium (VWR International SA) and were visualized with a Zeiss Axiovision fluorescence microscope. Prolactin (500 ng/mL, Sigma) was used to stimulate proliferation for 48 h. A minimum of 800 cells were counted per condition.

Stoll L., Rodríguez-Trejo A., Guay C., Brozzi F., Bayazit M.B., Gattesco S., Menoud V., Sobel J., Marques A.C., Venø M.T., Esguerra J.L., Barghouth M., Suleiman M., Marselli L., Kjems J., Eliasson L., Renström E., Bouzakri K., Pinget M., Marchetti P, & Regazzi R. (2020). A circular RNA generated from an intron of the insulin gene controls insulin secretion. Nature Communications, 11, 5611.

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