Nonidet P40 was purchased from Roche (Warszawa, Poland). Acrylamide, APS, bisAcrylamide, 0.5 M Tris pH 6.8 buffer, 1.5 M Tris pH 8.8 buffer, Laemmli Sample Buffer, 2-mercaptoethanol, TEMED, Tris-glycine buffer, Tris-glycine-SDS buffer were procured from Bio-Rad (Warszawa, Poland). PageRuler Prestained Protein Ladder was obtained from Fermentas (Thermo Fisher Scientific, Warszawa, Poland). DPBS and FBS were purchased from Life Technologies (Warszawa, Poland). 2,5-dihydroxybenzoic acid (2,5-DHB) was from Bruker Daltonics (Bremen, Germany). Acetonitrile HPLC grade for far UV, 2-aminobenzamide (2-AB), anthranilic acid (2-AA), sodium cyanoborohydride, DMSO and trifluoroacetic acid (TFA) were purchased from Sigma-Aldrich (Poznań, Poland). All other salts, alcohols and acids were analytical grade chemicals from Sigma-Aldrich. Water used was of Milli-Q grade.
2 5 dihydroxybenzoic acid 2 5 dhb
Manufactured by Bruker
Sourced in Germany
2,5-dihydroxybenzoic acid (2,5-DHB) is a chemical compound used as a matrix material in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. It facilitates the ionization and desorption of analyte molecules, enabling their detection and analysis by mass spectrometers.
Automatically generated - may contain errors
Lab products found in correlation
Trifluoroacetic acid tfa, by Merck Group (2 mentions)
2 mercaptoethanol, by Bio-Rad (1 mentions)
Bis acrylamide, by Bio-Rad (1 mentions)
Nonidet p 40, by Roche (1 mentions)
Dulbecco phosphate buffered saline (dpbs), by Thermo Fisher Scientific (1 mentions)
Acrylamide, by Bio-Rad (1 mentions)
Tris glycine sds buffer, by Bio-Rad (1 mentions)
Pageruler prestained protein ladder, by Thermo Fisher Scientific (1 mentions)
Temed, by Bio-Rad (1 mentions)
Tris glycine buffer, by Bio-Rad (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Laemmli sample buffer, by Bio-Rad (1 mentions)
Anti rabbit secondary antibody, by Abcam (1 mentions)
D glucose, by Merck Group (1 mentions)
Sequencing grade modified trypsin, by Promega (1 mentions)
Quikchange lightning site directed mutagenesis kit, by Agilent Technologies (1 mentions)
Cytidine, by Merck Group (1 mentions)
Guanosine, by Merck Group (1 mentions)
Adenosine, by Merck Group (1 mentions)
4 protocols using 2 5 dihydroxybenzoic acid 2 5 dhb
1
Protein Electrophoresis and Mass Spectrometry
2
Recombinant Kinase Protein Assay
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Active proteins (IKKα, IKKβ and Aurora kinase C; Signalchem, Vanier Place Richmond, BC, Canada), kinase buffer I and ATP stock solution were purchased from signalchem. Halo-Tag plasmid vector (pFN18A), Restriction enzyme blend (SgfI & PmeI), KRX expression cell, L-rhamnose monohydrate and sequencing-grade modified trypsin were purchased from Promega (Madison, WI). Quikchange lightning site-directed mutagenesis kit was purchased from Agilent technologies. D- glucose and phosphoric acid (PA) were purchased from Sigma-Aldrich. 2,5-Dihydroxybenzoic acid (2,5-DHB) was purchased from Bruker Daltonics. Acetonitrile (ACN, ultrapure ACS reagent grade and methyl alcohol (high purity HPLC reagent) were purchased from USB corporation and samchun pure chemical. All other chemicals used were of ACS or HPLC grade. Primary antibody (anti-IκBα monoclonal antibody) and anti-mouse secondary antibody were purchased from Santa Cruz Biotechnology and Cell signaling Technology. Primary antibody (anti-phospho-IκBα serine 32; Cell Signaling Technology, CA, USA, serine 63 and serine 262 monoclonal antibody; Custom made antibody) and anti-rabbit secondary antibody were purchased from abcam and Cell signaling Technology. Primer and DNA sequencing data were purchased cosmo genetech (Seoul, Korea).
3
Comprehensive Biochemical Reagents Protocol
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l -Aspartic acid, l -glutamic acid, l -histidine, l -phenylalanine, l -tyrosine, l -tryptophan,
thymidine, cytidine, uridine,
adenosine, guanosine, and trifluoroacetic acid (TFA) were purchased
from Sigma-Aldrich (St. Louis, MO). Peptides including Tyr–Gly–Gly
(YG-3), Tyr–Phe (YF-2), and Phe–Gly–Phe–Gly
(FG-4) were purchased from Shanghai Apeptide Co. Ltd. (Shanghai, China).
Glutathione, glucose, sucrose, and raffinose were obtained from J&K
Scientific Co. Ltd. (Beijing, China). TS-1 was supplied by XFNano,
Inc. (Nanjing, China). Dopamine hydrochloride, tris(hydroxymethyl)aminomethane
(Tris), sodium dihydrogen phosphate (NaH2PO4), disodium hydrogen phosphate (Na2HPO4), sodium
hydroxide (NaOH), sodium chloride (NaCl), and fatty acids (FAs), including cis-10-heptadecenoic acid (17:1), α-linolenic acid
(18:3), linoleic acid (18:2), trans-vaccenic acid
(18:1), and arachidonic acid (20:4), were purchased from Aladdin (Shanghai,
China). Matrices including 2,5-dihydroxybenzoic acid (2,5-DHB) and
α-cyano-4-hydroxycinnamic acid (CHCA) were purchased from Bruker
Daltonics GmbH (Bremen, Germany). Ethanol, mEthanol, and acetonitrile
(ACN) were purchased from Merck (Darmstadt, Germany). Bond Elut phenylboronic
acid (PBA) columns were obtained from Varian (Agilent, Santa Clara,
CA). Ultrapure deionized water (18 MΩ) used in all experiments
was prepared by a Milli-Q water purification system (Millipore, Bedford,
MA).
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thymidine, cytidine, uridine,
adenosine, guanosine, and trifluoroacetic acid (TFA) were purchased
from Sigma-Aldrich (St. Louis, MO). Peptides including Tyr–Gly–Gly
(YG-3), Tyr–Phe (YF-2), and Phe–Gly–Phe–Gly
(FG-4) were purchased from Shanghai Apeptide Co. Ltd. (Shanghai, China).
Glutathione, glucose, sucrose, and raffinose were obtained from J&K
Scientific Co. Ltd. (Beijing, China). TS-1 was supplied by XFNano,
Inc. (Nanjing, China). Dopamine hydrochloride, tris(hydroxymethyl)aminomethane
(Tris), sodium dihydrogen phosphate (NaH2PO4), disodium hydrogen phosphate (Na2HPO4), sodium
hydroxide (NaOH), sodium chloride (NaCl), and fatty acids (FAs), including cis-10-heptadecenoic acid (17:1), α-linolenic acid
(18:3), linoleic acid (18:2), trans-vaccenic acid
(18:1), and arachidonic acid (20:4), were purchased from Aladdin (Shanghai,
China). Matrices including 2,5-dihydroxybenzoic acid (2,5-DHB) and
α-cyano-4-hydroxycinnamic acid (CHCA) were purchased from Bruker
Daltonics GmbH (Bremen, Germany). Ethanol, mEthanol, and acetonitrile
(ACN) were purchased from Merck (Darmstadt, Germany). Bond Elut phenylboronic
acid (PBA) columns were obtained from Varian (Agilent, Santa Clara,
CA). Ultrapure deionized water (18 MΩ) used in all experiments
was prepared by a Milli-Q water purification system (Millipore, Bedford,
MA).
4
Mass Spectrometry Sample Preparation
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Milli-Q water (MQ) used in this study was generated from a Q-Gard 2 system (Millipore, Amsterdam, Netherlands), maintained at ≥18 MΩ. Ethanol, trifluoroacetic acid (TFA), sodium dodecyl sulfate (SDS), PBS (disodium hydrogen phosphate dihydrate (Na2HPO4 × 2H2O), potassium dihydrogen phosphate (KH2PO4) and sodium chloride (NaCl)) were purchased from Merck (Darmstadt, Germany). 1-hydroxybenzotriazole (HOBt) hydrate, sodium hydroxide, Nonidet P-40 (NP-40) and 1-ethyl-3-(3-(dimethylamino)propyl)-carbodiimide (EDC) hydrochloride were obtained from Sigma-Aldrich. Additional components used for this study included recombinant peptide-N-glycosidase F (PNGase F) from Roche Diagnostics (Mannheim, Germany), 2,5-dihydroxybenzoic acid (2,5-DHB) from Bruker Daltonics (Bremen, Germany) and HPLC Supra Gradient acetonitrile (ACN) from Biosolve (Valkenswaard, Netherlands).
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