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3 protocols using goat anti rabbit and anti mouse igg hrp

1

Immunostaining Protocol for Cellular Markers

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Example 8

Reagents. Antibodies used: Ki67 and keratin 6 (NovoCastra); PCNA (PC10) and αSMA (alpha-SM1) (Santa Cruz Biotechnology); CD31 (BD Pharmingen); CD68 (FA-11) (Biolegend); F4/80 (AbD Serotec); cANGPTL4: monoclonal antibodies against the C-terminal mouse (190-410 amino acids) of ANGPTL4 were produced by ProSci, respectively; goat anti-rabbit and anti-mouse IgG-HRP (Santa Cruz Biotechnology); Alexa Fluor 488 or 594 goat anti-mouse IgG, anti-rat IgG and anti-rabbit IgG (Molecular probes). DAB peroxidase substrate kit (Vector Laboratories). Unless mentioned otherwise, all chemicals were from Sigma-Aldrich and molecular biology enzymes from Fermentas. All oligonucleotides were synthesized by Sigma-Proligo.

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2

Autophagy Regulation in Neural Cells

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All reagents we used were commercially available. Fetal bovine serum (FBS) and Dulbecco's modified Eagle's medium (DMEM) were purchased from Invitrogen (Carlsbad, California). Recombinant human bFGF was purchased from Sigma (Sigma‐Aldrich, St. Louis, Missouri). Anti‐GFAP, anti‐bFGF, anti‐p62, anti‐NeuN, and anti‐GAPDH antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, California). Anti‐GAP43, anti‐LC3, anti‐Beclin‐1, and anti‐Nestin antibodies were purchased from Abcam (CB, United Kingdom). Goat anti‐rabbit and anti‐mouse IgG‐HRP, goat anti‐chicken IgY H&L, donkey anti‐goat IgG H&L were purchased from Santa Cruz Biotechnology. An enhanced chemiluminescence kit and CM‐DiI were purchased from Bio‐Rad (Hercules, California). Thapsigargin (TG) and 3‐methyladenine (3‐MA) were purchased from Sigma‐Aldrich. The autophagy activator rapamycin (RAPA) was purchased from Cell Signaling Technology. All other reagents were purchased from Beyotime Institute of Biotechnology (Shanghai, China) unless otherwise specified.
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3

Antibody Panel for Immunohistochemistry

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Example 8

Reagents.

Antibodies used: Ki67 and keratin 6 (NovoCastra); PCNA (PC10) and αSMA (alpha-SM1) (Santa Cruz Biotechnology); CD31 (BD Pharmingen); CD68 (FA-11) (Biolegend); F4/80 (AbD Serotec); cANGPTL4: monoclonal antibodies against the C-terminal mouse (190-410 amino acids) of ANGPTL4 were produced by ProSci, respectively; goat anti-rabbit and anti-mouse IgG-HRP (Santa Cruz Biotechnology); Alexa Fluor 488 or 594 goat anti-mouse IgG, anti-rat IgG and anti-rabbit IgG (Molecular probes). DAB peroxidase substrate kit (Vector Laboratories). Unless mentioned otherwise, all chemicals were from Sigma-Aldrich and molecular biology enzymes from Fermentas. All oligonucleotides were synthesized by Sigma-Proligo.

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